Survivin基因在NB4细胞株细胞和急性早幼粒细胞白血病细胞表达及其抗凋亡作用与临床意义  

Expression of Survivin Gene in NB4 Cell Line and Cells of Acute Promyelocytic Leukemia and Its Anti-apoptosis and Clinical Significance

作　　者：薛军[1] 林茂芳[2] 

机构地区：[1]南京医科大学附属南京市第一人民医院血液科,南京210006 [2]浙江大学医学院附属第一医院血液科,杭州310003

出　　处：《中国实验血液学杂志》2006年第2期332-336,共5页Journal of Experimental Hematology

摘　　要：为了检测survivin基因在APL细胞中的表达率并探讨其表达与临床的相关性,本研究采用RT-PCR方法检测PML/RARα和survivinmRNA表达。结果显示:NB4细胞经过ATRA处理后,survivinmRNA表达随着时间的延长而逐渐下降,在72小时几乎检测不到survivinmRNA表达。36例初发、复发APL患者骨髓单个核细胞均有PML/RARα融合基因表达,其中survivinmRNA阳性表达24例(占67%),阴性表达12例(占33%);22例缓解期APL患者骨髓单个核细胞PML/RARα融合基因表达均为阴性,其中survivinmRNA阳性表达8例(占36%),阴性表达14例(占64%);初发、复发组、PML/RARα基因长型阳性组与缓解组相比,survivinmRNA表达阳性率均明显增高(两者P值均<0.05),而与急性白血病组相比survivinmRNA表达阳性率均明显减低(两者P值分别<0.05,<0.001)。36例初发、复发APL患者,无论survivinmRNA表达阳性或阴性,用ATRA治疗都能达到完全缓解;临床上并发DIC和严重感染的4例APL患者的survivinmRNA表达皆为阳性(其中1例死亡),而survivinmRNA表达阴性患者临床症状均较轻,只有轻微的皮肤粘膜出血、发热和乏力等症状。2例APL患者经用ATRA治疗后外周血象以及骨髓象诱导分化症象不明显者,其survivinmRNA表达均为阳性。结论:APL患者骨髓单个核细胞sur-vivin基因阳性表达率较其它类型急性白血病明显减低,survivin基因表达与临床有一定的相关性。The study was aimed to detect expression rate of survivin gene in APL cell and to explore the relationship between its expression and clinical manifestation. PML/RARα and survivin mRNA expression were analyzed by using reverse transcriptase polymerase chain reaction （ RT-PCR ） technique. The results showed ： （ 1 ） the survivin gene expression was detected in NB4 cell line. By treatment with ATRA , survivin mRNA expression in NB4 cell gradually decreased along with time delay and almost could not be detected at the 72th hour. （2） the positive and negative rate of survivin mRNA expression was 67% and 33% respectively, while in all 36 cases of de novo and relapse APL patients, the PML/RAR fusion gene expression was positive. In 22 cases at remission stage, the PML/RARet fusion gene expression was negative, and the positive and negative rate of survivin mRNA expression was 36% and 64% respectively. The survivin mRNA expression positive rates in the de novo group, relapse group and PML/RARα fusion gene L-type positive group were obviously higher than those in remission period group （ P 〈 0.05 ） and were significantly lower than those in acute leukemia group （ P 〈 0.05, 〈 0. 001 ）. （ 3 ） whether the survivin mRNA expression was positive or negative in 36 cases of de novo and relapse APL patients, all the 36 cases could obtain complete remission. 4 APL patients with positive expression of survivin mRNA had DIC and serious infection （ one patient died）. The clinical symptom showed slight skin or mucosa bleeding, fever and asthenic in the patients with negative expression of survivin mRNA. When 2 APL patients with positive expression of survivin mRNA had been treated with ATRA, induction differentiation sign in their peripheral blood and bone marrow figures was not obvious. It is concluded that the survivin gene positive expression rate is lower in acute promyelocytic leukemia than that in any other types of leukemia and is related to clinical manifestation.

关 键 词：细胞株 NB4 SURVIVIN基因 PML/RARΑ融合基因 急性早幼粒细胞白血病 

分 类 号：R733.71[医药卫生—肿瘤]