非小细胞肺癌组织中Skp2的表达及其与p27^(kip1)和Ki-67蛋白表达的关系  

Expression of Skp2 in non-small cell lung cancer and its relationship with p27^(kip1) and Ki-67 protein expressions

作　　者：曾艳[1] 朱润庆[1] 马华玲[1] 夏和顺[1] 夏东[1] 黄娟[1] 

机构地区：[1]武汉大学医学院病理学教研室,湖北武汉430071

出　　处：《中华肿瘤防治杂志》2006年第2期93-96,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金资助项目(39870305)

摘　　要：目的:探讨Skp2的表达在非小细胞肺癌(nonsmallcelllungcancer,NSCLC)发生发展中的作用,及其与p27kip1和Ki67蛋白表达的关系。方法:应用免疫组化SP法检测Skp2、p27kip1和Ki67三种蛋白在60例NSCLC和20例正常支气管黏膜上皮组织中的表达。结果:NSCLC组织中Skp2蛋白表达的阳性率为48.33%(29/60),显著高于正常支气管黏膜上皮组织中的表达,P=0.000。Skp2的表达与肿瘤的组织学类型、肿瘤细胞的分化程度、TNM分期、淋巴结转移和患者吸烟与否显著相关,P值分别为0.038、0.005、0.019、0.010和0.002,但与患者的年龄及性别无关,P值分别为0.833和0.281。NSCLC组织中Skp2表达与p27kip1表达呈显著负相关,P=0.001;而与Ki67表达呈显著正相关,P=0.027。结论:Skp2在NSCLC组织中表达是上调的,可能是通过作用于细胞周期调控蛋白p27kip1,加速了对p27kip1泛素化依赖的蛋白降解,使其表达及代谢发生异常,导致细胞周期失控并促进细胞异常增殖,从而参与了NSCLC的发生和发展。OBJECTIVE：To investigate the expression of Skp2 and its relationship with expressions of p27^kip1 and Ki-67 proteins in human non-small cell lung cancer（NSCLC）. METHODS： Immunohistochemical methods were applied to detect the expressions of Skp2, p27^kip1 and Ki-67 proteins in 60 surgical specimens from NSCLC patients and 20 normal bronchial epithelium. RESULTS： In NSCLC, the positive rate of Skp2 protein was 48. 33% （29/60）. Which was obviously higher than that in normal bronchial epithelium, P = 0. 000. There was no relationship between Skp2 expression and age （P = 0. 833） or sex （P=0.281）,respectively;otherwise, there was closely rdationship between Skp2 expression and histological subtype, cellular differentiation, TNM stage, lymph node metastasis, smoking, P= 0. 038, 0. 005, 0. 019, 0. 010 and 0. 002, respectively. Expression of Skp2 was negatively correlated with expression of p27^kip1 , P = 0. 001 ; but was positively correlated with expression of Ki-67, P = 0. 027. CONCLUSlONS： Expression of Skp2 is up-regulated significantly in NSCLC compared with normal bronchial epithelium, and overexpression of Skp2 reduces the protein level of p27^kip1 through ubiquitin-dependent degradation and causes disruption of cell cycle control and enhancement of the proliferative activity, indicating Skp2 playes an important role in oncogenesis and development of NSCLC.

关 键 词：癌 非小细胞肺/病理学 S期激酶相关蛋白类/遗传学 Ki-67抗原/生物合成 细胞周期蛋白质依赖激酶类/代谢 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]