阻断c-kit对小鼠小肠Cajal间质细胞的影响及机制初探  

Exploring the Effect of Blockade of c-kit on Interstitial Cells of Cajal and its Mechanisms in the Balb/c Murine Small Intestine

作　　者：贾后军[1] 刘宝华[1] 童卫东[1] 张连阳[1] 文亚渊[1] 刘韧[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所普通外科,重庆400042 [2]第三军医大学大坪医院野战外科研究所第二研究室

出　　处：《结直肠肛门外科》2006年第1期6-9,共4页Journal of Colorectal & Anal Surgery

基　　金：国家自然科学基金资助项目(30300156)

摘　　要：目的:观察阻断c-kit对小鼠小肠Cajal间质细胞(ICC)的影响以及与Cajal间质细胞c-kit基因表达的关系。方法:选取新出生Balb/c小鼠,腹腔内隔天注射c-kit抗体(ACK2)100μg,共5次,对照组注射生理盐水,于小鼠出生后第10天观察小肠Cajal间质细胞的分布情况并检测小肠肌条c-kitmRNA及蛋白的表达。结果:阻断c-kit后小鼠小肠中未能明显观察到ICC分布,并且c-kitmRNA表达以及c-kit蛋白表达明显减少。结论:阻断c-kit可导致小鼠小肠Cajal间质细胞缺失,与ICCc-kit基因水平表达下调引起ICC发育受影响有关。Objective：To investigate the effect of blockade of c-kit on interstitial cells of Cajal in the Balb/c routine small intestine. Methods. New-born routine were injected intraperitoneally antibody of c-kit（ACK2 ） 100μg every second day, and control groups were injected saline. The distribution of ICC was observed by immunohistochemistry and the expressions of c-kit were measured by RT-PCR and Western Blot when the routine were 10 days postpartum. Results：The distribution of ICC was lost and the mRNA and protein expression of c-kit were significantly lower when c-kit were blocked. Conclusion： When the c-Kit signal is blocked, ICC loss is found and the mechanisms of which may be related with c-kit downregulated.

关 键 词：CAJAL间质细胞 c—kit 小肠 小鼠 蛋白表达 

分 类 号：R574[医药卫生—消化系统]