结直肠中ING1甲基化的性别不一致性研究  

Sexual Discordant Patterns of ING1 Promoter Methylation in Colorectal Cancer

作　　者：曹云飞[1] 陈利生[1] 高枫[1] 

机构地区：[1]广西医科大学第一附属医院结直肠肛门外科,南宁530021

出　　处：《结直肠肛门外科》2006年第1期29-32,共4页Journal of Colorectal & Anal Surgery

基　　金：广西科学基金(桂科回0342018);教育部留学回国人员基金(教外司留2004-176)

摘　　要：目的:检测结直肠癌中是否存在ING1启动子甲基化,ING1甲基化和性别的关系。方法:用甲基化特异性PCR方法检测62例结直肠癌患者的肿瘤组织、相应的正常黏膜的ING1的甲基化状况。结果:ING1甲基化在62份肿瘤标本中有26例发生了甲基化(41.9%),而在相应的正常组织中只有4例(6.45%)。DukesC、D期患者癌组织的甲基化发生率55.6%明显高于DukesA、B期(23.1%)。在肿瘤组织中ING1甲基化表现出明显的性别和年龄差异(P<0.05)。结论:结直肠癌中ING1的甲基化不仅和肿瘤的分期有关,而且可由年龄与性别相关的因素调节,可能是结直肠癌发生过程中的早期事件。Objective：To investigate the methylation of ING1 promoter in primary colorectal cancer and to assess the sexual discordant patterns of ING1 methylation in colorectal cancer. Methods：We analyzed ING1 methylation in tumor tissues, paired normal tissue from 62 colorectal cancer patients and their clinical characteristics. The methylation stafus of ING1 was determined by methylation-specific polymerase chain reaction. Results：ING1 methylation was detected in 26 （41.9%） of 62 tissue samples and in 4 （6.45%） of 62 matched normal tissues. The incidence of hypermethylaiotn of ING1（55. 6%） in the cancerous tissues of the patients in DukesC and D was significantly higher than that in Dukes A and B（6.45%）. ING1 methylation showed an age and gender bias in the tumor tissues（ P〈0.05）. Conclusion：ING1 methylation not only may correlate significantly with the progression of colorectal cancer, but also occurs at the early stage of colorectal cancer and may be regulated by age- and gender-specific factor.

关 键 词：ING1 结直肠癌 性别 甲基化 年龄 

分 类 号：R735.3[医药卫生—肿瘤]