神经胶质瘤组织中p19和mdm2蛋白的表达及相关性研究  

Expressions and their relationship between p19 and mdm2 protein in gliomas

作　　者：梁君[1] 曹英海[1] 刘程伟[1] 

机构地区：[1]中国医科大学附属第一医院神经外科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2006年第5期354-356,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的：探讨p19和mdm2基因在神经胶质瘸发生、发展中的可能作用及二者的相关性。方法：应用免疫组化SP法对66例神经胶质瘸组织中的p19和mdm2蛋白表达水平进行检测。结果：p19蛋白在神经胶质瘤Ⅰ级阳性率为57．89％，Ⅱ级阳性率为26．92％，Ⅲ～Ⅳ级阳性率为4．76％，Ⅰ与Ⅲ～Ⅳ级阳性率比较，差异有统计学意义，x^2=13．41，P〈0．01；Ⅰ与Ⅱ级和Ⅱ与Ⅲ～Ⅳ级阳性率比较，差异无统计学意义。mdm2蛋白在神经胶质瘸Ⅰ级阳性率为5．26％，Ⅱ级阳性率为30．77％，Ⅲ～Ⅳ级阳性率为66．67％，Ⅰ与Ⅲ～Ⅳ级阳性率比较，差异有统计学意义，x^2=16．05，P〈0．01；Ⅰ与Ⅱ级和Ⅱ与Ⅲ～Ⅳ级阳性率比较，差异无统计学意义。p19和mdm2的阳性表达呈负相关性。结论：p19和mdm2基因可能参与了神经胶质瘸的发生发展过程，其蛋白的检测有可能作为判定其恶性程度的一个辅助指标。OBJECTIVE：To investigate the role and their relationship between p19 and mdm2 genes on the growth and development of gliomas. METHODS： The sensitive and differential SP method was used to detect the expressions of p19 and mdm2 protein in 66 gliomas. RESULTS： The positive rates of p19 were 57. 89% in grade Ⅰ, 26. 92% in Ⅱ and 4. 76% in Ⅲ -Ⅳ. The positive rates were compared between grade Ⅰ and Ⅲ- Ⅳ, the difference was significant, x^2 = 13. 41, P〈 0. 01. The positive rates were compared between grade Ⅰ and Ⅱ and between Ⅱ and Ⅲ- Ⅳ, the differences were not significant. The positive rates of mdm2 in grade Ⅰwere 5. 26%, 30. 77% in Ⅱ and 66. 67% in Ⅲ-Ⅳ. The positive rates were compared between grade Ⅰand Ⅲ-Ⅳ, the difference was significant, x^2 = 16. 05, P 〈 0. 01. The positive rates were compared between grade Ⅰ and Ⅱ and between Ⅱ and Ⅲ-Ⅳ, the differences were not significant. There was an inverse relationship between p19 and mdm2 expressions. CONCLUSION： p19 and mdm2 gene probably involves in the occurrence and development of gliomas and the detection of the two kinds of protein may act as assistant diagnosis items for determinations malignant degree of tumor.

关 键 词：癌与抑癌基因蛋白质/生物合成 神经胶质瘤/病理学 免疫组织化学 

分 类 号：R730.2[医药卫生—肿瘤]