肿瘤坏死因子-α诱导人恶性胶质瘤U251细胞核因子-κB活化及其意义  

TNF-α induced activation of NF-κB in U251 human malignant glioma cells and its significance

作　　者：陈海锋[1] 程远[2] 游潮[1] 黄爱龙[3] 

机构地区：[1]四川大学华西医院神经外科,四川成都610041 [2]重庆医科大学第二附属医院神经外科,重庆400012 [3]重庆医科大学病毒性肝炎研究所,重庆400012

出　　处：《中华神经外科疾病研究杂志》2006年第2期111-114,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30070297)

摘　　要：目的研究肿瘤坏死因子-α(TNF-α)对人恶性胶质瘤U251细胞核因子-κB(NF-κB)的诱导活化作用,并观察NF-κB抑制剂对TNF-α诱导U251细胞凋亡的影响。方法采用电泳迁移率变动分析法检测TNF-α对U251细胞NF-κB的诱导活化,并通过甲基噻唑蓝(MTT)法和流式细胞技术检测NF-κB抑制剂对TNF-α诱导U251细胞凋亡的影响。结果TNF-α对U251细胞NF-κB具有诱导活化作用,其作用效果具有时间依赖性。NF-κB抑制剂对TNF-α诱导的U251细胞凋亡具有明显的促进作用(P<0.01)。结论TNF-α对胶质瘤细胞中的NF-κB具有诱导活化作用,抑制NF-κB活化可以提高胶质瘤细胞对TNF-α诱导的凋亡作用的敏感性。Objective To study tumor necrosis factor-α （TNF-α） induced activation of nuclear factor-κB （NF-κB） in U251 human malignant glioma cells and to observe the influence of inhibitor of NF-κB on TNF-α induced apoptosis of U251 cells.Methods The activation of NF-κB induced by TNF-α in U251 cells was examined by electrophoretic mobility shift assay. The influence of NF-κB inhibitor on TNF-α induced apoptosis of U251 cells was examined by methyl thiazolyl tetrazolium （MTF） and flow cytometry （FCM） method. Results TNF-α could induce activation of NF-κB in U251 cells with a time-related fashion. TNF-α induced apoptosis of U251 cells were obviously enhanced by NF-κB inhibitor （P〈0.01）.Conclusion TNF-α leads to the activation of NF-κB in glioma cells and the inhibition of NF-κB activation can increase the sensitivity of glioma cells to TNF-α induced apoptosis.

关 键 词：肿瘤坏死因子-Α 核因子-ΚB 胶质瘤 凋亡 

分 类 号：R730.264[医药卫生—肿瘤]