大鼠胰肠引流式单纯胰腺移植动物模型的建立(英文)  

Establishment of models of pancreas transplantation alone with entericdrainage in rats

作　　者：刘小南[1] 霍婷婷[2] 王为忠[1] 管文贤[1] 

机构地区：[1]解放军第四军医大学西京医院胃肠外科,陕西省西安市710033 [2]解放军第四军医大学西京医院肝胆外科,陕西省西安市710033

出　　处：《中国临床康复》2006年第17期174-176,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:单纯胰腺移植主要适用于病程尚未发展严重并发症的糖尿病患者。建立稳定的大鼠单纯胰腺移植动物模型对移植后免疫耐受和缺血再灌注损伤的研究非常重要。目的:建立大鼠胰肠引流式单纯胰腺移植的动物模型。设计:分组对照动物实验。单位:解放军第四军医大学西京医院胃肠外科。材料:90只SD雄性大鼠(250～320g)。糖尿病大鼠模型采用自阴茎静脉注射脲链霉素65mg/kg,空腹血糖持续2周超过19.4mmol/L为模型成功,58只大鼠静脉注射脲链霉素后,共有22只成功。大鼠分为2组:10只正常大鼠为对照组;22只糖尿病大鼠予单纯胰腺移植,22只正常大鼠为供体。方法:实验于1999-01/2004-07在第四军医大学西京医院胃肠外科实验室进行。单纯胰腺移植组血管吻合采用供胰腺腹主动脉绊(含腹腔动脉及脾动脉)及门静脉绊(含脾静脉)与受体腹主动脉及左肾静脉分别行端侧和端端吻合(袖套式);胰腺断端与小肠行Roux-y式吻合。主要观察指标:于术前2d和术后1,3,7,14,30d监测受体体质量、进食量、饮水量和空腹血糖,并分析失败原因。结果:模型组大鼠22只,正常大鼠10只,均进入结果分析。①大鼠静脉注射脲链霉素后有22只产生了较为严重的糖尿病症状,体质量、饮食量、饮水量及空腹血糖均较正常大鼠增加。②供体平均手术时间为(32.2±12.7)min,受体平均手术时间为(63.4±15.9)min,移植物均无热缺血时间,冷缺血时间为(48.6±18.3)min。除11例于1个月内死亡或胰腺失去功能外,均成活1个月以上。术后并发症主要为继发性胰腺炎和胰漏(7例,31.8%)。成功的单纯胰腺移植后受体第1天血糖即下降(P<0.01),第3天基本达到正常水平;饮水量、进食量、尿量均减少(P<0.01),第14天后基本稳定。结论:该试验方法可以建立相对稳定的动物模型,成功的单纯胰腺移植均可有效地改善糖尿病大鼠内分泌功能。BACKGROUND： Pancreas transplantation alone （PTA） is an effective therapy for diabetic patients who do not occur chronic complications. It＇s important to establish the stable PTA animal models to investigate immunologic tolerance or iscbemic/repedusion injury. OBJECTIVE： To establish the model of pancreas transplantation alone （PTA） with enteric drainage in rat. DESIGN： Grouping and controlled animal experiment. SETTING： Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA . MATERIALS： Totally 90 SD male rats, with the body mass of 250-320 g, were chosen in this study. 58 rats were induced by intravenous administration of streptozotocin （STZ） at a dose of 65 mg/kg via penile vein and the rats whose fasting plasma glucose exceeded 19.4 mmol/L for more than 2 weeks were selected, 22 rats was successful. Rats were randomly assigned to 2 groups： control group （10 healthy rats） and group PTA consisted of 22 diabetic rats, which received PTA from 22 normal donors. METHODS： This experiment was conducted at the laboratory of Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA from January 1999 to July 2004. The blood vessels reconstruction of PTA were performed using end-to-side anastomosis between the donors＇ abdominal aorta segment （abdominal artery and splenic artery） and recipients＇ abdominal aorta, and end-to-end anastomosis between the donors＇ portal vein segment （splenic vein） and recipients＇ left renal vein （use a cuff）. Pancreas exocrine drainage was made by pancreas intestine anastomosis （Roux-Y）. MAIN OUTCOME MEASURES： Body mass, food intake, water intake and fasting blood glucose were monitored 2 days before operation and 1,3, 7,14 and 30 days after operation, and the failure causes were analyzed. RESULTS： 22 rats in the model group and 10 rats in the normal control group entered the stage of result analysis. ①After injection of

关 键 词：胰腺移植 动物模型 引流术 大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]