绵羊急性缺血性左心衰竭模型的建立  

Establishment of Acute Ischemic Left Ventricular Heart Failure Model in Sheep

作　　者：李智成[1] 陈长志[1] 叶清[1] 成少飞[1] 王维俊[1] 汤敏[1] 赵晓刚[1] Y.John Gu 

机构地区：[1]上海第二医科大学附属仁济医院胸心外科,上海200127 [2]荷兰Groningen大学医院胸心外科

出　　处：《中国胸心血管外科临床杂志》2006年第2期102-105,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：中荷合作课题;仁济医院专项基金资助项目

摘　　要：目的建立简易可靠、相对稳定、重复性好的绵羊急性缺血性左心衰竭模型。方法14只雌性绵羊,体重36.80±3.43kg。用部分阻断冠状动脉左回旋支中段结合快速心脏起搏的方法建立急性左心衰竭模型。观察绵羊心力衰竭前、后血流动力学改变,心肌大体和电子显微镜下病理改变。结果10只绵羊心力衰竭诱导成功。绵羊心力衰竭模型建立后心排血量从3.74±0.48L/min降低至2.02±0.51L/min(P<0.01),平均动脉压从116.10±14.15mmHg(1kPa=7.5mmHg)降低至68.10±14.72 mmHg(P<0.01),中心静脉压从7.10±2.18mmHg升高至10.70±3.50mmHg(P<0.05),右心室舒张期末压从6.10±3.57mmHg升高至9.90±4.41mmHg(P<0.05),左心房压从8.10±2.13mmHg升高至12.00±4.57mmHg(P<0.01),左心室舒张期末压从8.50±4.17mmHg升高至13.10±10.64mmHg(P>0.05)。心肌超微结构损伤明显。结论部分阻断冠状动脉左回旋支中段结合快速心脏起搏建立急性左心衰竭模型,方法简便易行、相对稳定、重复性好,可用于评估心脏辅助装置的辅助效果。Objective To report an acute ischemic left ventricular heart failure model of safe, simple, relatively steady, and reproducible in sheep. Methods Fourteen female sheep with a body weight of 36.80±3.43kg were used in this study. Heart failure model was induced by partial occluding the middle left circumflex coronary artery （LCX） combined with pacemaker-induced tachycardia. Hemodynamic measurement was done before and after heart failure, myocardial examination was observed. Results Heart failure model was induced successfully in 10 sheep. Cardiac output dropped from 3.74±0.48L/min to 2.02±0. 51L/min （P〈0. 01）, mean arterial pressure decreased from 116. 10± 14.15 mmHg（1kPa = 7.5mmHg） to 68. 10± 14. 72mmHg （P〈0.01）, central venous pressure rose from 7. 10±2.18mmHg to 10. 70± 3.50 mmHg （P〈0.05）, right ventricular end-diastolic pressure increased from 6.10±3.57mmHg to 9.90±4.41mmHg（P〈0.05）, left atrial pressure increased from 8.10±2.13 mmHg to 12.00± 4.57mmHg （P〈0. 01 ）, and left ventricular end-diastolic pressure increased from 8. 50± 4. 17mmHg to 13.10± 10. 64mmHg（P〉 0. 05）. The myocardial ultrastructure injuries was marked. Conclusions Acute ischemic left ventricular heart failure could be induced by partial occlusion of the middle LCX combined with pacemaker-induced taehyeardia in sheep. This model is simple, easy to manipulate, relatively steady, and reproducible . It may be used for assessing cardiac assist devices.

关 键 词：急性心力衰竭 大动物 模型 心肌梗死 心室起搏 

分 类 号：R-332[医药卫生]