COX-2和NET-1基因蛋白在宫颈癌中表达的意义  

The Significance of Expression of COX-2 and NET-1 Gene Protein in Cervical Cancer

作　　者：张璇[1] 陈莉[2] 晓霄[1] 顾晓梅[1] 鄂群[2] 

机构地区：[1]江苏南通大学附属医院妇产科,226001 [2]南通大学病理教研室

出　　处：《肿瘤防治研究》2006年第4期252-254,F0003,共4页Cancer Research on Prevention and Treatment

摘　　要：目的 研究环氧合酶-2（COX-2）和NET-1基因蛋白在宫颈癌中的表达及与宫颈癌生物学行为的关系。方法 采用免疫组化SP法，检测COX-2和NET-1基因蛋白在50例宫颈鳞癌组织、36例宫颈上皮内瘤样病变（CINⅠ～Ⅲ级）及5例正常宫颈组织中的表达。结果 COX-2表达在宫颈浸润癌中明显高于C烈Ⅲ级和早期浸润癌（P〈0．01），而在CINⅢ级与早期浸润癌中无显著差异（P〉0．05）；COX-2阳性表达在低、中分化癌中均显著高于高分化癌（P〈0．01）；在淋巴结转移组中显著高于无淋巴结转移组（P〈0．01）。NET-1在CINⅢ级中表达显著高于CINI级和CINⅡ级（P〈0．01）。而CINⅢ级和宫颈癌比较无显著差异（P〉0．05）。NET-1阳性表达在淋巴结转移组明显高于无淋巴结转移组（P〈0．01）。宫颈癌中COX-2表达与NET-1表达呈正相关（P〈0．01）。结论 COX-2表达上调是宫颈癌演进中的常见的分子事件。可作为判断宫颈癌淋巴结转移的一个指标。NET-1的过表达可能是宫颈癌发生的早期分子事件，有可能成为宫颈癌早期诊断的组织标记。在宫颈癌中COX-2和NET-1基因蛋白之间有协同作用。Objective To study the expression of COX-2 and NET-1 gene protein in cervical cancer,and investigate the relationship between the expression and biologic behavior of cervical cancer. Methods Immunohistochemical SP method was used to detect the expression of COX-2 and NET-1 in 50 cases of cervical invasive squarnous cell carcinornas,36 cases of cervical intraepithelial neoplasias（CINⅠ～Ⅲ ） and 5 cases of normal cervical tissues. Results The positive expression rate of COX-2 in invasive carcinoma of cervix was significantly higher than that in CINⅢ（P〈0. 01 ） and early invasive carcinoma of cervix （P〈0. 01）,but there was no significant difference in expression of COX-2 between CINⅢ and early invasive carcinoma of cervix（P〉0. 05）. The positive expression rate of COX-2 in poor and moderate differentiate carcinoma was respectively higher than that in well-differentiated carcinoma （P〈0. 01）. In addition, the expression of COX-2 also significantly associated with lymph node metastasis （P〈0. 01）. The positive expression rate of NET-1 in CINⅢ was significantly higher than that in CIN Ⅰ （P〈0. 01） and CINⅡ （P d0. 01）. But there was no significant difference in NET-1 expression between CINⅢ and cervical cancer （P〉0. 05）. NET-1 expression was closely correlated with lymph node metastasis（P〈0. 01）. COX-2 expression was significantly correlated with NET-1 expression （P〈0. 01）. Conclusion COX-2 expression upregulated may be a common molecule event in the occurrence and development of cervical cancer and may be used as a marker of estimating lymph node metastasis. The overexpression of NET-1 may be an early gene event in the occurrence of cervical cancer and may become an early diagnostic marker in cervical cancer. The expression of COX-2 and NET-1 has a coordinate role.

关 键 词：环氧合酶-2(Cox-2) NET-1 宫颈癌 癌生物学行为 

分 类 号：R737.33[医药卫生—肿瘤]