大鼠肝癌细胞与I型胶原的黏附特性及其与细胞周期的关系  

Mechanical Properties of Rat HCC Adhesion to Collagen I and Its Relationship with Cell Cycle

作　　者：宋关斌[1] 秦建[1] 严润彬[1] 申晓东[1] 罗庆[1] 蔡绍皙[1] 孙才新[2] 

机构地区：[1]重庆大学生物工程学院生物力学及组织工程教育部重点实验室,重庆400044 [2]重庆大学电气工程学院高电压及电工新技术教育部重点实验室,重庆400044

出　　处：《生物医学工程学杂志》2006年第2期313-317,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(19972077and10372121);中国博士后科学基金资助项目(20040350155)

摘　　要：肿瘤细胞和胞外基质之间的黏附特性与肿瘤的侵袭转移有密切关系。作者从细胞周期的角度,采用微管吸吮技术和细胞同步技术研究了不同细胞周期肝癌细胞与I型胶原裱衬表面的黏附力学特性。结果表明:胸腺嘧啶脱氧核苷、秋水仙碱顺序阻断和胸腺嘧啶脱氧核苷双阻断后释放培养的方法可分别获得G 1期和S期肝癌细胞,平均同步率分别为74.09%和90.39%;在研究剂量和时间范围内,肝癌细胞与I型胶原的黏附力具有浓度和时间依赖性;S期肝癌细胞和I型胶原的黏附力值与G 1期和未同步组(对照组)相应值比较明显降低。结果提示:肝癌细胞经血道转移的侵蚀细胞间质阶段,G 1期细胞可能起更重要的作用。这一研究对全面认识肝癌的转移机理有重要意义。The mechanical properties of tumor cells adhering to extracellular matrix（ECM） are closely related with their invasion and metastesis. In this study we investigated the adhesive mechanical properties between hepatocellular carcinoma cells （HCC） and the collagen Ⅰ coated surfaces from the viewpoint of cell cycle by coupling cellular biology and cellular mechanics, using micropipette aspiration and cell synchronization technique. The results showed that the synchronous G1 and S phase HCC cells were achieved by use of thymine-2-desoryriboside, colchicines sequential blockage method and double thymine-2-desoryriboside blockage method, and that the synchronous rates of G1 and S phase HCC amounted to 74.09~ and 90. 39~ respectively. Within the ranges of dosing and timing in this study, the adhesion of HCC cells to collagen Ⅰ displayed dose dependent and time dependent patterns. S phase cells had small force of adhesion to collagen Ⅰ as compared with G1 phase and controlled cells （P〈0. 001）, which suggested that G1 phase HCC may play an important role in the step of invading interstitial connective tissue in the metastasis pathway of HCC through blood circulation. These are of significance to unveiling the mechanism of HCC metastasis.

关 键 词：肝肿瘤 细胞周期 Ⅰ型胶原 黏附 微管吸吮技术 

分 类 号：R735.7[医药卫生—肿瘤] R681[医药卫生—临床医学]