CO_2气腹对荷瘤大鼠IL-1β、IL-6和腹膜巨噬细胞功能的影响  

Effects of CO_2 pneumoperitoneum on levels of interleukin-1β and interleukin-6,and peritoneal macrophages in tumor-bearing rats

作　　者：郭庆军[1] 裘正军[1] 刘俊[1] 王瑞涛[1] 钟福全[1] 祝哲诚[1] 

机构地区：[1]上海市第一人民医院普外科

出　　处：《中国微创外科杂志》2006年第4期306-308,共3页Chinese Journal of Minimally Invasive Surgery

摘　　要：目的采用种植性大鼠肝脏肿瘤模型观察CO2气腹对细胞因子和腹膜巨噬细胞功能的影响。方法荷瘤大鼠32只随机分为4组(n=8):对照组(仅麻醉)、开腹组、免气腹组和CO2气腹组。术后2、24 h收集血清,检测血清中的IL-1β、IL-6水平;术后48 h,收集培养腹膜巨噬细胞,检测巨噬细胞产生的TNF-α水平。结果术后2、24 h开腹组IL-6的水平分别为(57.92±2.06)pg/m l,(35.49±1.15)pg/m l,显著高于CO2气腹组(14.64±0.34)pg/m l,(15.39±0.86)pg/m l,免气腹组(24.75±1.53)pg/m l,(17.10±0.97)pg/m l和对照组(17.75±1.60)pg/m l,(14.55±0.25)pg/m l(P<0.05)。术后2 h开腹组IL-1β的水平为(92.63±4.81)pg/m l,显著高于CO2气腹组(57.94±4.46)pg/m l、免气腹组(58.48±3.20)pg/m l和对照组(58.99±2.30)pg/m l(P<0.05)。CO2气腹组腹膜巨噬细胞产生的TNF-α水平为(35.69±3.54)pg/m l,显著低于免气腹组(68.87±4.08)pg/m l、开腹组(82.96±5.39)pg/m l和对照组(66.45±7.96)pg/m l(P<0.05)。结论腹腔镜术后机体应激反应较小,CO2气腹可能抑制腹膜巨噬细胞的功能。Objective To investigate effects of CO2 pneumoperitoneum on cytokines and peritoneal macrophages in a rat model with implanted liver tumor. Methods A total of 32 Wistar rats with implanted liver tumor were randomly divided into 4 groups （ n = 8） ： Control Group （ anesthesia only） , Laparotomy Group, Gasless Group （ gasless laparoscopy）, and Pneumoperitoneum Group （laparoscopy under CO2 pneumoperitoneum）. Serum samples were collected at the 2nd and 24th hours after the procedure respectively for the detection of levels of interleukin-1β （IL-1β） and interleukin-6 （IL-6）. Samples of peritoneal maerophages were collected and incubated for the detection of levels of tumor necrosis factor-or （TNF-α） , a product of macrophages. Results At the 2nd and 24th hours after surgery, levels of serum IL-6 in the Laparotomy Group （57.92±2.06 pg/ml and 35.49 ± 1.15 pg/ml） were significantly greater than those in the Pneumoperitoneum Group （ 14.64 ± 0.34 pg/ml and 15.39 ± 0.86 pg/ml）, the Gasless Group （24.75 ± 1.53 pg/ml and 17.10±0.97 pg/ml）, and the Control Group （17.75±1.60 pg/mland 14.55 ±0.25 pg/ml） （P〈0.05）. Levels of serum IL-1β at the 2nd postoperative hours were significantly higher in the Laparotomy Group （92.63 ± 4.81 pg/ml） than in the Pneumoperitoneum Group （57.94 ± 4.46 pg/ml） , the Gasless Group （58.48 ± 3.20 pg/ml） , and the Control Group （58.99 ± 2.30 pg/ml） （P 〈 0.05 ）. Levels of TNF-α from peritoneal macrophages were significantly lower in the Pneumoperitoneum Group （35.69 ± 3.54 pg/ml） than in the Gasless Group （68.87 ± 4.08 pg/ml）, the Laparotomy Group （82.96 ± 5.39 pg/ml）, and the Control Group （66.45± 7.96 pg/ml） （ P 〈 0.05 ）. Conclusions During laparoscopic surgery the systemic stress reaction is not distinctive. The carbon dioxide pneumoperitoneum may suppress functions of peritoneal macrophages.

关 键 词：IL-1Β IL-6 巨噬细胞 TNF—α 

分 类 号：R735.7[医药卫生—肿瘤]