慢病毒载体感染终末分化骨髓瘤细胞的研究  

Study on infection of nondividing myeloma cells by lentivirus vector

作　　者：邓少丽[1] 胡福泉[1] 蹇锐[1] 饶贤才[1] 蒋静[1] 程小星[1] 

机构地区：[1]第三军医大学基础医学部微生物学教研室,重庆400038

出　　处：《中国现代医学杂志》2006年第7期978-980,共3页China Journal of Modern Medicine

基　　金：国家自然科学基金(30370603)资助

摘　　要：目的研究慢病毒载体有效转移并表达于终末分化骨髓瘤细胞。方法构建表达GFP(绿色荧光蛋白)及NeoR(新霉素抗性基因)的慢病毒载体,分别感染多发性骨髓瘤细胞J558L。采用荧光显微镜及流式细胞仪检测GFP的表达,用G418筛选携NeoR慢病毒载体感染的J558L细胞。结果包装的慢病毒滴度达6×105IU/mL,对J558L细胞的感染效率为5%￣10%。通过G418筛选可得到稳定长期表达neo基因的阳性细胞克隆。结论慢病毒载体可有效感染终末分化细胞,是有发展潜力的基因治疗新载体。[Objective] To study the efficient and stable transduction of nondividing myeloma cells J558L by lentivirus vector. [Methods] The lentiviras vector to express EGFP （Enhance green fluorescence protein） and NeoR （Neomycin resistance gene） were constructed respectively. The expression of EGFP was observed by fluorescence microscope and flow cytometer. The J558L cells affected by lentivirus were screened by G418. [Results] The titer of lentivirus was 6×10^5（P IU （Infected Unit）/mL, transduction efficiency ranged from 6～11%. The J558L cells affected by lentivirus could express NeoR gene stably. [Conclusion] The nondividing J558L cell could be transducted efficiently by lentivirus vectors, Lentivirus is an efficient vector of gene therapy on hematological system disease.

关 键 词：慢病毒载体 骨髓瘤细胞 基因治疗 

分 类 号：R733.3[医药卫生—肿瘤]