郁金中姜黄素类化合物的毛细管区带电泳法测定  

Separation and Determination of Curcumins in Curcma aromatica salisb by Capillary Zone Electrophoresis

作　　者：薛玲[1] 林秀丽[2] 张惠云[1] 主沉浮[3] 

机构地区：[1]山东中医药大学中药学院,山东济南250014 [2]山东大学药学院,山东济南250012 [3]山东大学化学与化工学院,山东济南250100

出　　处：《化学研究与应用》2006年第4期368-371,共4页Chemical Research and Application

基　　金：山东省中青年科学家科研奖励基金(03BS046)

摘　　要：本文在提取中药郁金中姜黄素类化合物的基础上,利用毛细管区带电泳法对郁金中所含黄素类化合物进行了分离研究,确定了最佳分离条件:9mmol/L单-6-O-苯基氨甲酰基-β-CD(mono-6-O-phenylcarbamoyl-β-CD),40mmol/L硼砂缓冲液,分离电压为15kV,毛细管柱温度为22℃,紫外检测波长为214nm,pH值为9.0。双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素在此条件下10 m in内能够达到基线分离,方法快速、简便、且消耗试剂少,不污染环境。A capillary zone electrophoresis（CZE） method was developed for the determination and separation of curcumin, demethoxy curcumin and bis-demethoxy curcumin in the Chinese herbal extract in Curcuma aromatica salisb from Shandong, Jinan, Detection at 214 nm with a system containing sodium borate buffer and mono-6-O-phenlcarbamoyl-β-CD was found to be the most snitable approach for this analysis, The three curcumins could easily be determined within 10 minutes by this method. The effect of pH value, the concentration of the running buffer and mono-6-O-phenlcarbamoyl-β-CD on the migration time and resolution of the curcumins were also discussed.

关 键 词：郁金 姜黄素类化合物 高效毛细管区带电泳 

分 类 号：O657.8[理学—分析化学]