利用烟草表达一种新型鲑鱼降钙素的初步研究  

A preliminary study on the expression of a new type salmon calcitonin gene in transgenic tobacco

作　　者：王晓晔[1] 普颖颖[1] 宋杰[1] 王国丰[1] 钱虹妹[1] 林娟[2] 赵凌侠[1] 唐克轩[1] 

机构地区：[1]上海交通大学植物生物技术研究中心复旦-交大-诺丁汉植物生物技术研究中心,上海200240 [2]复旦大学遗传工程国家重点实验室,上海200433

出　　处：《扬州大学学报（农业与生命科学版）》2006年第1期9-13,共5页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家高技术研究发展计划项目(8632002AA206511);上海市重大科技攻关项目(03DZ19310)

摘　　要：利用烟草表达一种新型鲑鱼降钙素，以期为利用植物系统表达鲑鱼降钙素的研究提供理论参考。首先构建含外源基因“pil-msCT”的植物双元表达载体p35S-2300-twin T—DNAs：pil-msCT：NOS—ter，然后通过农杆菌介导法转化烟草，获得卡那霉素抗性烟草128株，其中PCR阳性植株14株（阳性率10．94％）；Southern blotting分析表明外源基因已整合到烟草基因组中，拷贝数为2～8个；半定量RT—PCR结果显示。转基因烟草株间“pil-msCT”转录水平存在较大差异。Salmon calcitonin （sCT） has been proved to be an effective therapeutic peptide for Osteoporosis. In this study, tobacco was transformed with a modified gene msCT so as to produce a new type of sCT. Firstly, the plant binary expression vector p35S-2300-twinT-DNA ： pil-msCT ： NOS-ter containing foreign gene ＂pil-msCT＂ was constructed; Then, the fusion gene ＂pil-msCT＂ was introduced into tobacco plants by Agrobacterium-medlated transformation. 128 kanamycin resistant tobacco plants were regenerated, in which 14 were confirmed by PCR analysis （positive rate 10.94 % ）; Southern blot analysis proved that the copies of gene pil-msCT in tobacco plants ranged from 2 to 8 ; Semi-quantitative RT PCR detection showed different transcript levels of foreign gene among independent plants of To generation.

关 键 词：骨质疏松症 突变型鲑鱼降钙素 转基因烟草 

分 类 号：Q786[生物学—分子生物学] R589[医药卫生—内分泌]