超临界CO_2萃取益智油及益智油的抗氧化活性  

Extraction of Aplinia Oxyphylla Miquel Seed Essence via Supercritical Carbon Dioxide and Antioxidant Activity of the Extracts

作　　者：刘红[1] 郭祀远[1] 肖凯军[1] 蔡妙颜[1] 韩长日[2] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]海南师范学院化学系,海南海口571158

出　　处：《华南理工大学学报（自然科学版）》2006年第3期54-57,共4页Journal of South China University of Technology(Natural Science Edition)

基　　金：海南省自然科学基金资助项目(20301)

摘　　要：运用超临界CO2分离技术萃取益智仁中的抗氧化性物质,以l,1-二苯基-2-苦基苯肼(DPPH)清除率和过氧化值为检验指标,采用气相色谱-质谱联用技术分析超临界CO2萃取的益智油的化学成分.分析结果表明,在40℃,15MPa下分离萃取的益智油中含有诺卡酮、益智酮A和益智酮B.不同条件萃取的益智油对花生油的抗氧化性顺序为:40℃,15MPa下的萃取油>2,6-二叔丁基对甲酚(BHT)>35℃,25MPa下的萃取油>50℃,12MPa下的萃取油>35℃,25MPa(乙醇夹带)下的萃取油.超临界CO2萃取所得的益智油对DPPH的清除率明显高于水蒸气萃取油,其中,40℃和15MPa下所得的萃取油对DPPH的清除率最高;高温萃取油对DPPH的清除率有所降低.The antioxidant substances in Aplinia OxyphyUa Miquel seed were extracted by supercritical carbon dioxide （ SCCO2 ）, and the chemical compositions of the extracts were determined by means of GC-MS, with the scavenging rate for 1,1 -diphenyl-2-picrylhydrazyl （DPPH） radical and the peroxide value for the assessment of antioxidant actions. The results indicate that nootkatone, yakuchinone A and yakuchinone B can be extracted at 40 ℃ and 15 MPa, that the antioxidant activity of the extracts obtained in varied conditions on peanut oil are as follows： the extracts at 40 ℃ and 15 MPa 〉 butylated hydroxytoluene （BHT） 〉 the extracts at 35 ℃ and 25 MPa 〉 the extracts at 50 ℃ and 12 MPa 〉 the extracts at 35 ℃ and 25 MPa （ adding ethanol）, that the extracts obtained by SCCO2 extraction are of greater scavenging rate for DPPH than that obtained by hydrodistillation extraction, especially the ex- tracts at 40 ℃ and 15 MPa, and that the scavenging rate decreases with the increase in extraction temperature.

关 键 词：超临界CO2 萃取 益智油 抗氧化活性 

分 类 号：TQ028.32[化学工程]