高效表达非融合蛋白的大肠杆菌质粒载体的构建  

CONSTRUCTION OF PLASMID VECTORS EXPRESSING UNFUSED PROTEINS WITH HIGH EFFICIENCY IN E. COLI

作　　者：马大龙[1] 劳者歌 孙英[1] 庞建 宋泉声[1] 

机构地区：[1]北京医科大学免疫学教研室

出　　处：《北京医科大学学报》1990年第2期81-83,共3页Journal of Peking University(Health Sciences)

基　　金：国家863计划资助

摘　　要：利用基因重组技术构建了高效表达非融合蛋白的大肠杆菌质粒载体pKPL系列。它们含有PL启动子、MS-2聚合酶SD顺序、起始密码、多克隆点及rrnB终止子顺序。利用pKPL-2D构建的PL-CAT-3质粒高效表达氯霉素转乙酰酶(Cat),表达量占菌体蛋白的30％以上。用pKPL质粒还表达出有生物活性的人白细胞介素-3和人粒单集落刺激因子。A new series of plasmid vectors pKPL was constructed by DNA recombinant techniques. Thesevectors express unfused proteins with high efficiency in E. coli. They contain the initiator ATG, themultiple cloning sites(MCS)and rrnB terminator. The plasmid pLCAT-3, which was derived from pKPL-2D, synthesizes chloramphnicol acetyltransferase (Cat) more than 30% of the total cellular protein.In addition, the pKPL series was successfully used for expressing human interleukin - 3 (IL-3) andGM-colony stimulator (GM-CSF) with biological activities.

关 键 词：基因工程 大肠杆菌 表达载体 

分 类 号：Q78[生物学—分子生物学]