超短波和旋磁对局灶性脑缺血再灌注损伤的影响  

The effect of ultrashortwave diathermy and rotating magnetic therapy on cerebral ischemia and reperfusion

作　　者：梁维娣[1] 张志强[1] 

机构地区：[1]中国医科大学第二临床医院康复科,沈阳110004

出　　处：《中华物理医学与康复杂志》2006年第4期225-228,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：辽宁省社会发展基金资助项目(No.99225003)

摘　　要：目的探讨超短波、旋磁治疗对大鼠局灶性脑缺血再灌注损伤的影响。方法应用线栓法制作大鼠局灶性脑缺血再灌注模型。将52只健康Wistar大鼠随机分为假手术组、对照组、超短波治疗组(超短波组)及旋磁治疗组(旋磁组)。脑缺血再灌注后18h给予治疗,24h取脑,观察各组大鼠脑组织含水量、脑梗死体积及Bcl-2、Bax蛋白表达,进行组间比较。结果脑缺血再灌注后18h,超短波治疗能减少脑含水量,减小梗死体积;超短波组与旋磁组Bcl-2的表达均提高,Bax蛋白表达降低,Bcl-2/Bax提高,超短波组与旋磁组间差异无统计学意义(P>0.05);在减少脑含水量和减小脑梗死体积方面,旋磁组与对照组比较,差异无统计学意义(P>0.05)。结论脑缺血再灌注后18h,应用超短波治疗对局灶性脑缺血再灌注损伤有显著疗效;本实验条件下旋磁治疗对改善脑水肿、减小脑梗死体积未见显著疗效,在改善凋亡方面有显著疗效。Objective To investigate the effect and mechanism of ultrashortwave（ USW ） diathermy and rotating magnetic（ RM ）field therapy on the brain following transient focal cerebral ischemia and reperfusion in rats. Methods Fifty-two Wistar rats were randomly divided into a sham operation group, an ischemia and a reperfusion control group, a USW treatment group and a RM treatment group. Focal cerebral ischemia and reperfusion were induced by intraluminal filament occlusion of the middle cerebral artery. Each brain was removed at 24 h after the reperfusion and water content, cerebral infarct volume and histological expression of Bcl-2 and Bax proteins were observed. Results When USW treatment was started at 18 h after reperfusion, decreased infarct volume and water content were observed, but RM treatment did not show this relationship. Both USW and RM were associated with in- creased expression of Bcl-2 protein and decreased the expression of Bax protein. Conclusions USW treatment is beneficial in focal cerebral ischemia. RM＇s effect is uncertain. Any effect is through reducing apoptosis.

关 键 词：脑缺血再灌注 超短波 旋磁 脑梗死 凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]