药物干预对帕金森病大鼠行为的影响  

Behaviour Change in Rats with PD after Drug Administration

作　　者：蔡秀英[1] 孔小明[1] 费娜[1] 孔岩[1] 刘春风[2] 

机构地区：[1]苏州大学附属第一医院,江苏苏州215006 [2]苏州大学附属第二医院,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2006年第2期196-199,共4页Suzhou University Journal of Medical Science

摘　　要：目的通过建立大鼠帕金森病(PD)异动症(LID)模型,观察大鼠在药物干预前后的行为改变。方法选取内侧前脑束(MFB)注射6-OHDA建立单侧毁损PD鼠模型,给予左旋多巴(L-dopa 20 mg.kg-1.d-1)及生理盐水(NS,2 ml)干预满1个月,并设正常对照。观察大鼠的行为并每周1次阿朴吗啡诱发旋转行为,每2两次异常不自主运动(AIM)评分。结果MFB注射点造模成功率为75%,PD大鼠的行为改变有一定的规律性,其旋转速度存在先升后降的现象;AIM评分显示异动症发生率为72%。结论选择MFB注射点造模成功率高;PD大鼠行为变化有规律性;LID的发生与波动用药、损伤程度密切相关。Objective To investigate the effect of one-month intermittent levodopa（L-dopa） administration on behaviour in rats with Parkinsonism and establish the rat model of L-dopa induced dyskinesias（LID）. Methods Unilateral 6-OHDA lesioned rat model of Parkinson＇s disease（PD） was developed by injecting 6-OHDA in the Medial forebrain bundle（MFB）. Those successful PD rats were divided into two groups（A＆B）. The rat received L-dopa（A） or saline（B） injection once daily for 1 month, respectively. The behaviour change was observed, abnormal involuntary movement（AIM）was written down and apomorphine induced rotation was counted. Results Most rats were proved to be successful by apomophine inducting. The ratio is 75 %. Rats with PD had behaviour change in the treatment, the rotation counts increased from the first 2 to 3 weeks and then decreased gradually. In A group 72 % of them developed AIM. Conclusions Through MFB point injection the rat model in PD is developed in high ratio. Rats with PD have behaviour characterization in our observation. The pulsatile L-dopa administration and the severity in rats may be involved in the formation of LID.

关 键 词：帕金森病 左旋多巴 旋转行为 异动症 

分 类 号：R742.5[医药卫生—神经病学与精神病学]