孕激素对绒癌细胞系Galectin-3表达的影响  

Effect of progestogen on galectin-3 expression in choriocarcinoma cell line

作　　者：郭慧[1] 钟刚[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科内分泌实验室,430030

出　　处：《中国优生与遗传杂志》2006年第4期25-27,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的通过体外试验研究孕激素对BeWo绒癌细胞系中Galectin-3蛋白的表达,探讨其对滋养细胞侵袭性相关基因表达的影响作用。方法体外培养BeWo绒癌细胞,分别加入不同浓度的孕激素24h后,采用免疫印迹技术、免疫荧光技术测定各种条件下的Galectin-3蛋白的表达。结果Bewo绒癌细胞系表达Galectin-3。分别用0、10-7、10-6、10-5mol/L孕激素处理24h后,10-7、10-6mol/L的孕激素可以显著提高BeWo绒癌细胞系中Galectin-3蛋白的表达(P<0.05,相反10-5mol/LP使Galectin-3表达降低(P<0.05)。结论(1)BeWo绒癌细胞系有Galectin-3的表达。(2)低浓度的孕激素可以诱导Galectin-3蛋白的表达。(3)高浓度的孕激素对Galectin-3蛋白的表达有下调作用。Objective： To show the effect of progesterone on the invasiveness - associated gene expression in choriocarcinoma cells. The effect of P on Galectin - 3 expression in BeWo choriocarcinonla cells line was detected in vitro. Methods ： BeWo choriocareinoma cells were incubated with progesterone （0 M, 10^-7 M, 10^-6 M, 10^-5 M） for 24 hours, respectively. The expression of Galeetin -3 protein detected by in immunofluorescence and western bloting. Results： Galectin -3 protein expression in BeWo cells incubated with 10^-7 M, 10^-6 M progesterone increased （ P 〈0. 05 ）. However 10^-5 M progesterone could significantly decrease the expression of Galeetin -3 protein （ P 〈0. 05）, Conclusion： （ 1 ） There was expression of Galeetin -3 protein in BeWo choriocareinoma cells. （2） Low density progesterone could elevate the level of Galectin- 3 protein. （3） High density progesterone could down -regulate the expression of Galectin -3 protein in BeWo choriocarcinoma cells.

关 键 词：GALECTIN-3 孕激素 BeWo绒癌细胞 

分 类 号：R737.33[医药卫生—肿瘤]