葛根素对血管性痴呆大鼠海马中低氧诱导因子-1α和红细胞生成素表达的影响  

Effects of puerarin on the expression of hypoxia inducible factor-1α and erythropoietin in the hippocampus of vascular dementia rats

作　　者：吴海琴[1] 张蓓[1] 张桂莲[1] 展淑琴[1] 张茹[1] 赵英贤[1] 

机构地区：[1]西安交通大学医学院第二附属医院神经内科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2006年第2期132-136,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关资助项目(No.2002K10-G2)

摘　　要：目的观察葛根素对血管性痴呆(VD)大鼠海马中低氧诱导因子-1α和红细胞生成素表达的影响并探讨其可能机制。方法采用大鼠双侧颈总动脉永久性阻断法(2-VO)建立VD的动物模型,SD大鼠随机分为假手术组、2-VO组和葛根素组,每组又分为1周、3周、6周、2月(n=6)四个时间点;应用Y型水迷宫实验测定动物的学习记忆能力,采用免疫组化法检测大鼠海马CA1区HIF-1α和EPO蛋白的表达。结果①2-VO组和葛根素组大鼠游全程时间和误入盲端次数均显著增加,但各时间点葛根素组大鼠的学习记忆成绩均显著优于2-VO组。②各时间点,2-VO组和葛根素组大鼠海马CA1区HIF-1α和EPO的表达均较假手术组明显增高,但葛根素组两种蛋白的表达均较2-VO组明显降低。结论葛根素可提高缺血脑组织细胞内氧浓度,改善学习记忆功能,对VD具有确切的保护作用。Objective To observe the effects of puerarin on the expression of hypoxia inducible factor-1α and erythropoietin in the hippocampus of vascular dementia rats and to investigate its possible mechanisms. Methods Rats of experimental group were treated with a permanent bilateral common carotid arteries （CCA） occlusion （2-VO） to establish vascular dementia model. Male SD rats were randomly divided into three groups： sham-operated group, 2-VO group and puerarin group（twenty-four rats in each group）. Rats were evaluated on learning-memory ability by Y-type water maze test. At corresponding time points at lw, 3w, 6w and 2mon, the dynamic expression of HIF-1α and EPO in CA1 region of hippocampus was determined by immunohistochemical method. Results ① The swimming time and error times of rats in 2-VO group and puerarin group were significantly increased; but at all time points the rats in puerarin group showed better performance than those in 2- VO group. ② Both in 2-VO group and puerarin group, the number of HIF-1α and EPO positive cells in hippocampal CA1 region were much more than that in sham-operated group; but the expression of HIF-1 α and EPO at each point in puerarin group was distinctly less than that in 2-VO group. Conclusion Puerarin can increase the intracellular oxygen concentration and improve learning-memory ability during cerebral ischemia, and it has definitely protective effects on VD.

关 键 词：葛根素 血管性痴呆 低氧诱导因子-1Α 促红细胞生成素 

分 类 号：R743.3[医药卫生—神经病学与精神病学]