AKT2、HIF-1α在胰腺癌中的表达及其临床意义  

EXPRESSION AND CLINICAL SIGNIFICANCE OF AKT2 AND HIF-1α IN PANCREATIC CANCER

作　　者：杨鹏[1] 王春友[1] 牛彦锋[1] 万赤丹[1] 魏海燕[1] 徐建波[1] 罗威[1] 杨明[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2006年第2期178-181,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助项目(30571817)

摘　　要：目的探讨胰腺癌组织中丝氨酸/苏氨酸蛋白激酶2(AKT2)、HIF-1α蛋白的表达及其临床意义。方法应用免疫组化SABC法检测63例胰腺癌组织和23例胰腺良性病变组织中AKT2、HIF-1α蛋白的表达,分析其与胰腺癌临床病理因素的关系。结果AKT2、HIF-1α蛋白在胰腺癌组织中的阳性表达率分别为39.7%(25/63)、63.5%(40/63),明显高于胰腺良性病变组的13%(3/23)、17.4%(4/23)(P<0.05)。AKT2表达与HIF-1α表达呈正相关(P<0.05)。AKT2表达与组织学分级、淋巴结转移、TNM分期有关(P<0.05),而与患者的性别、年龄、胰腺癌大小无关(P>0.05)。结论AKT2和HIF-1α在胰腺癌组织中表达增高,AKT2可能通过调节HIF-1α表达来影响胰腺癌的发生、发展、转移。Objective To investigate the expression and clinical significance of AKT2 and HIF-1α protein in human pancreatic carcinoma. Methods The expressions of AKT2 and HIF-1α protein were measured by using SABC immunohistochemical staining in specimens of 63 eases of pancreatic carcinoma and 23 eases of benign pancreatic diseases. The relationship of AKT2 and HIF-1α protein expressions with clinicopathological factors in pancreatic carcinoma was also analyzed. Results The positive expression rates of AKT2 （39. 7%） and HIF-1α （63. 5%） in pancreatic carcinoma were significantly higher than those in benign pancreatic diseases （P（0. 05）, respectively. The expression of AKT2 was positively correlated with that of HIF-1α protein （P（0. 05）. The expression level of Akt2 in pancreatic cancer showed an obvious correlation with histological grade, lymph node metastasis, and TNM stage （P〈0.05, respectively）, but not with the patient＇ s age, gender and tumor size. Conclusion Overexpressions of AKT2 and HIF-1α were found in pancreatic carcinoma. AKT2 may induce the carcinogenesis and aggression in pancreatic cancer by regulating the transcription of HIF-1α gene.

关 键 词：胰腺癌 丝氨酸/苏氨酸蛋白激酶 缺氧诱导因子1Α 免疫组织化学 

分 类 号：R735.9[医药卫生—肿瘤]