HPLC法测定血清肉碱的研究  

STUDY ON DETERMINATION OF CARNITINE IN SERUM BY HPLC

作　　者：冯一[1] 张顺国[1] 徐远飞[1] 周建德[2] 陶晔璇[2] 蔡威[2] 

机构地区：[1]上海第二医科大学附属新华医院临床营养中心,药剂科 [2]上海市儿科医学研究所,上海200092

出　　处：《营养学报》2006年第2期177-179,共3页Acta Nutrimenta Sinica

基　　金：国家"十五"科技攻关计划"优生优育"项目(No.2004BA709B09)

摘　　要：目的：建立高效液相色谱（HPLC）测定血清中肉碱浓度的方法。方法：血清样品用乙腈、甲醇沉淀蛋白，加入肉碱衍生剂，60℃恒温水浴2h后进样10μl。色谱柱为Atlantis C18柱（4．6mm×150mm，5μm），流动相为甲醇：异丙醇：乙腈（35：45：20，v／v），流速1．0ml／min，紫外检测波长260nm。结果：在上述色谱条件下，血清肉碱分离良好，回归方程为Y=8490X-48200，r=0．9999，线性范围为12．5～400μmol／L。最低检测浓度为12．5μmol／L。日内精密度1．6％～13．2％，日间精密度（％）1．8％～12．1％，回收率98．5％。结论：通过加入肉碱衍生剂，建立了HPLC法测定血清中肉碱浓度的方法。此法操作简单，耗时少，有良好的准确度，适用于临床检测。Objective： To develop a method for determination of carnitine in serum using HPLC. Methods： The serum proteins were precipitated by acetonitrile and methanol. The organic extract was added derivatizing reagent and the solution was warmed to 60℃ for 2 hours. Atlantis C18 column （4.6 mm×150 mm, 5μm） was used as stationary phase and methanol-ispropand-acetonitrile （35 ： 45 ： 20, v/v） as mobile phase; flow rate 1.0ml/min, UV wavelengh at 260nm. Results： The typical chromatogram from serum samples showed clear separation of carnitine. Y = 8490X-48200, r = 0.9999. Intra-day RSD was 1.6%-13.2%, and inter-day 1.8%-12.1%. The recovery rate was 98.5%. Conclusion： A method for determination of carnitine in serum using HPLC by adding derivatizing reagent was developed and it is simple, rapid and precise, and can be used for clinical test.

关 键 词：肉碱 血清 HPLC法 测定 

分 类 号：R446.1[医药卫生—诊断学]