压力对人眼小梁细胞自分泌TGF-β_2的影响  

Effect of Pressure on Autocrine of TGF-beta 2 in Human Trabecular Meshwork Cells

作　　者：张虹[1] 陈莲一[1] 李贵刚[1] 单长梅[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《华中科技大学学报（医学版）》2006年第2期241-243,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(2003ABA150)

摘　　要：目的 采用开放式压力控制培养系统研究压力对体外培养的人眼小粱细胞自分泌转化生长因子-β2（TGF-β2）的影响，探讨TGF-β2在原发开角型青光眼发病机制中的作用。方法 体外培养人眼小梁细胞并传至第5代。对照组不施加压力（0mmHg，1mmHg=0．133kPa），实验组施加20、40、60及80mmHg压力，分别持续0、12、24、36h。采用双抗体夹心酶联免疫吸附（ELISA）法测定细胞上清液中TGF-β2浓度。结果 小梁细胞自分泌TGF-β2随压力增高而增加，与对照组比较，差异有显著性意义（P〈0．05）；在20～60mmHg范围内，加压时间越长，TGF-β2增高越明显；虽然80mmHg压力水平下TGF-β2增高幅度下降，但加压12和24h组TGF-β2水平仍明显高于对照组（P〈0．05）。结论随压力增高，小梁细胞自分泌TGF-β2增多，其水平与压力大小及作用时问相关，推测TGP-β2分泌异常可能是原发开角型青光眼病理进程中的重要机制之一。Objective By using a new culture system, which could impose pressure on cells with open cycling air, to study the effects of pressure on autocrine of TGF-beta 2 in human trabecular meshwork cells in order to explore the role of TGF-beta 2 in the pathogenesis of primary open angle glaucoma. Methods The human trabeeular meshwork cells were cultured in vitro and transferred to the 5th passage for experiments. The new culture system could control the pressure at different levels and applied to impose pressures of 20, 40, 60 and 80 mmHg on each experiment group respectively. Furthermore, the cells had been cultured for 12, 24 and 36 h under each pressure, Those cells cultured without any pressure imposed were taken as the control group. The concentration of TGF-beta 2 was tested by enzyme-linked immunosorbent assay. Results The level of TGF beta 2 secreted by cells in experimental groups was increased as the pressure increased in comparison with that in control group （P〈0.05）. During a pressure range of 20-60 mmHg, the concentration of TGF-beta 2 was increased more obviously with the prolonged time of the pressure imposed on the cells. The cells cultured under 40 mmHg pressure for 36 h autocrined TGF-beta 2 with the highest level of （1305.36±105.33） pg/ml. The concentration of TGF-beta 2 in the 80 mmHg pressure group was lower than that in other experimental groups, but the TGF-beta 2 level in the 80 mmHg/12 h and the 80 mmHg/24 h groups was still significantly higher than that in the control group （P〈0.05）. Conclusions The increased pressure promoted the human trabeeular meshwork cells to autocrine TGF-beta 2 and the level was related to different pressure levels and their sustained times, These findings indicated the elevated coneentratlon of TGF beta 2 in aqueous humor of patients might play a role in the pathogenesis of primary open angle glaucoma.

关 键 词：转化生长因子-Β2 青光眼 小梁细胞 压力 

分 类 号：R775[医药卫生—眼科]