慢性髓系白血病急变期中免疫分型研究  

Study on the immunophenotype of blast crisis in chronic myeloid leukemia

作　　者：朱雨[1] 李建勇[1] 吴雨洁[1] 宋君红[1] 郑文娟[1] 杨慧[1] 张建富[1] 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院血液科,210029

出　　处：《白血病．淋巴瘤》2006年第2期84-86,共3页Journal of Leukemia & Lymphoma

基　　金：江苏省"135"医学重点人才基金资助项目(RC2002044)

摘　　要：目的探讨慢性髓系白血病急变期(CML-BC)的免疫表型特征及应用价值。方法采用一组单克隆抗体和三色流式细胞术对36例成年人CML-BC骨髓标本进行免疫表型分析。结果36例CML-BC患者中急性非淋巴细胞白血病变30例(83.33%),其中40%(12/30)伴淋系表达;急性淋巴细胞白血病变急淋变3例(8.33%),其中66.67%(2/3)伴髓系表达;急性混合型白血病变2例;急性未分化型白血病变1例。CML-BC以CD33阳性率最高(91.67%),其次是CD1+3(86.11%),CD3+4(61.11%),CD+7(33.33%),CD1+0(19.44%),CD1+9(16.67%),CD+2(2.78%),CD2+0(5.56%)及CD1+4(5.56%)。CD7与CD34共阳性27.78%。结论CML-BC免疫表型复杂,多系表达常见。免疫分型可协助判断CML的急变类型。Objective To characterize the immunophenotype of chronic myeloid leukemia in blast crisis （CML-BC）. Methods lmmunophenotypic analysis was performed using a panel of monoclonal antibodies and three-colour immunofluorescence staining methods of flow cytometry in 36 patients with CML in BC. Results Of the 36 CML-BC, 30 patients （83.3 %） were acute nonlymphocytic leukemia （ANLL） transformation, 12 of them simultaneously expressed lymphoid associated antigens, 3 patients （8.33 %） were acute lymphoid leukemia （ALL） transformation and 2 of them simultaneously expressed myeloid associated antigens. Another 3 cases were classified as acute undifferentiated leukemia （AUL） and 2 hybrid acute leukemia （HAL）. Among the 36 CML-BC cases, CD33 was the most commonly expressed antigen in CML-BC： 91.67 %, followed by CDj3 86.11%, CD34 61.11%, CD7 33.33 %, CD10 19.44 %, CD19 16.67 %, CD2 2.78 %, CD200 5.56 %, CD14 5.56 %, respectively. CD34 and CD7 were coexpressed in 10 out of 36 cases. Conclusion The present study confirms the lineage heterogeneity of CML-BC, co-expression of markers from two or three lineages is frequent. Immunophenotyping is important in accurate classification of CML-BC.

关 键 词：慢性髓系白血病 免疫分型 

分 类 号：R733.7[医药卫生—肿瘤]