针刺预处理对全脑缺血大鼠脑组织细胞凋亡的影响  

Effect of acupuncture pretreatment on apoptosis in brain tissues of rats with global cerebral ischemia

作　　者：孙忠人[1] 张力[2] 唐伟[3] 李晓捷[4] 单丽莉[1] 马睿杰[1] 

机构地区：[1]黑龙江中医药大学附属第二医院神经内科,黑龙江省哈尔滨市150040 [2]黑龙江中医药大学2004级博士研究生班,黑龙江省哈尔滨市150040 [3]大连大学附属医院神经内科,辽宁省大连市116020 [4]佳木斯大学康复医学院,黑龙江省佳木斯市154003

出　　处：《中国临床康复》2006年第15期144-145,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察手针和电针预处理对全脑缺血大鼠脑组织细胞凋亡的影响,并与缺血预处理结果进行比较。方法:①实验于2005-02/2005-9在黑龙江中医药大学实验中心完成。①选用雄性健康Wistar大鼠108只。采用4-动脉阻断法复制大鼠全脑缺血模型。按随机抽签法将大鼠分为6组(每组18只):正常组(正常饲养,不予任何处置),假手术组(仅暴露双侧椎动脉及双侧颈总动脉),脑缺血组(热凝双侧椎动脉及动脉夹夹闭双侧颈总动脉10min),脑缺血预处理组(热凝双侧椎动脉及动脉夹夹闭双侧颈总动脉3min,24h后全脑缺血10min),手针预处理组(术前7d用0.25mm×40mm毫针针刺大鼠双侧足三里穴,直刺进针5mm;双侧曲池穴,直刺进针4mm,0.25mm×25mm毫针针刺百会穴,平刺约3mm。针刺预处理组间歇5min捻转一次,平补平泻,1次/d,干预30min,预处理后24h全脑缺血10min),电针预处理组(术前7d采用全能脉冲电疗仪,对足三里、曲池穴进行电针针刺,频率为1Hz,电压为2V,强度以肢体轻微抖动为度,1次/d,干预30min。预处理后24h全脑缺血10min)。②术后24,48,72h各组各处死鼠6只,取脑,采用细胞凋亡原位末端标记法检测大鼠脑组织的细胞凋亡情况。③组间计量资料比较采用方差分析。结果:大鼠108只均进入结果分析。大鼠大脑皮质及海马CA1区细胞凋亡数:脑缺血预处理组、手针预处理组、电针预处理组、假手术组、正常组明显少于脑缺血组(P<0.05);电针预处理组大鼠大脑皮质细胞凋亡数明显少于手针预处理组(P<0.05),与脑缺血预处理组相近。结论:针刺预处理可以通过抑制神经细胞凋亡,且电针干预效果与脑缺血预处理相近,优于手针干预后。AIM： To investigate effect of hand acupuncture and electro-acupuncture pretreatment on apoptosis in brain tissue of rats with global cerebral ischemia and compare with the effect of ischemie pretreatment. METHODS： The experiment was completed at the Experimental Center of Heilongiiang University of Traditional Chinese Medicine from February to September 2005. ① Totally 108 male health^r Wistar rats were selected in this study. Models of global cerebral ischemia of rats were copied with four vessels occlusion. All rats were divided into 6 groups with 18 in each group according to random lot method. Rats in normal group were fed normally and not treated with any methods. Rats in sham operation group were exposured bilateral vertebral artery and bilateral common carotid artery. Bilateral vertebral artery of rats in cerebral isehemia group was thermocoagulated and bilateral common carotid artery was clipped with artery clamp for 10 minutes. Bilateral vertebral artery of rats in cerebral ischemia pretreatment group was thermocoagulated and bilateral common carotid artery was clipped with artery clamp for 3 minutes. Twenty-four hours later, global cerebral ischemia was maintained for 10 minutes. Rats in hand acupuncture treatment group were aeupunctured at bilateral Zusanli vertically with milli-needle sized 0.25 mm × 40 mm in the depth of 5 mm 7 days before operation, at bilateral Quchi vertically in depth of 4 ram, and at Baihui flatly with milli-needle sized 0.25 mm× 25 mm in the depth of 3 mm. Needle was twirled once every 5 minutes, and uniform reinforcing-reducing method was interfered once a day for 30 minutes. Twenty-four hours after pretreatment, global cerebral ischemia was maintained for 10 minutes.Rats in electro-acupuncture pretreatment group were acupunctured at Zusanli and Quchi at frequency of 1 Hz and voltage of 2 V with omnipotent impulse electro-therapeutic apparatus 7 days before operation. Light tremble of limbs was regarded as the standard intensity. Acupuncture was interfered once a day

关 键 词：脑缺血/针灸疗法 针灸效应 细胞凋亡 

分 类 号：R742[医药卫生—神经病学与精神病学]