吩噻嗪衍生物协同放射对HeLa细胞增殖的抑制效应比较研究  

Suppressive effect on HeLa cells proliferation by phenothiazine derivatives alone and combining with ionizing radiation

作　　者：严雨倩[1] 闫宇邱 王林[1] 彭涛 隋建丽[1] 白贝[1] 孙伟建 周平坤[1] 

机构地区：[1]北京放射医学研究所,100850 [2]解放军三○七医院 [3]不详

出　　处：《中华放射医学与防护杂志》2006年第2期114-117,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家高技术研究发展计划(863)资助项目(2004AA221160);国家自然科学基金资助项目(30270423)

摘　　要：目的探讨吩噻嗪衍生物对人宫颈癌HeLa细胞增殖的抑制作用及与放射联合应用抑制肿瘤的协同效应。方法采用MTT法和细胞克隆形成法检测细胞的增殖活性和细胞辐射敏感性。结果比较了6种吩噻嗪衍生物对HeLa细胞增殖的抑制效应,发现化合物α氯N二甲胺基乙基吩噻嗪(PTZD2)、α三氟甲基N二甲胺基乙基吩噻嗪(PTZD3)和α氯N二乙胺基乙基吩噻嗪(PTZD5)的作用比较明显,在10μmol L浓度能产生出显著抑制效应,40～50μmol L浓度作用3和4d,细胞增殖完全被抑制而死亡。PTZD2或PTZD3与放射联合应用,显示出明显抗HeLa细胞增殖的协同效应,10μmol LPTZD3对2和4Gy照射细胞的增殖抑制的增强比分别为3.5和1.8。实验结果还表明,在照射前18h用药的协同作用更加显著。结论吩噻嗪衍生物具有程度不同的抑制HeLa细胞增殖的作用,与放射联合应用具有抗肿瘤的协同效应,而且照射前18h用药的效应最佳。Objective To examine the antiproliferative effects of phenothialzine derivatives （PTZDs） alone on HeLa cells and in combination with ionizing radiation. Methods MTT and colony-forming method were used to evaluate the proliferation activity and cellular radiosensitivity of HeLa cells. Results We compared the antiproliferative effects of six phenothialzine derivatives, and found that the derivatives α-chloro-N- dimethylamine phenothialzine （PTZD2）, α-triflumethyl-N- α dimethylamine ethyl） phenothialzine （ PTZD3 ） and α-chloro-N-（ dimethylamine ethyl） phenothialzine （PTZD5） showed a significant antiproliferative effect at concentration of 10 μmol/L. HeLa cells proliferation was completely suppressed when treated with PTZDs of 40-50 μmol/L. PTZD2/PTZD3 and colbat-60 gamma-irradiation showed synergistic suppressive effect on proliferation of HeLa cells. The enhancement ratios of 10 μmol/L PTZD3 combination with 2 Gy and 4 Gy irradiations were 3.5 and 1.8, respectively. The maximum synergistic suppressive effect was observed when cells administered with PTZD3 at 18 h before being irradiated. Conclusion Phenothialzine derivatives show antiproliferations on HeLa cells, and differ in degrees. The synergistic anticancer effect could be obtained by combining phenothialzine derivatives with radiotherapy.

关 键 词：电离辐射 吩噻嗪 细胞增殖 协同效应 HELA细胞增殖 吩噻嗪衍生物 

分 类 号：R737.33[医药卫生—肿瘤]