左旋四氢巴马汀对脑缺血/再灌注大鼠脑组织血红素氧化酶-1及内源性一氧化碳的影响  

Effects of L-tetrahydropalmatine on hemeoxygenase-1 in cerebral tissue of rats and endogenous carbon monoxide during global cerebral ischemia/reperfusion

作　　者：韩遵义[1] 杨光田[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,湖北武汉430030

出　　处：《中国中西医结合急救杂志》2006年第2期89-92,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：湖北省医药卫生科技计划项目(WJ01518)

摘　　要：目的:观察全脑缺血/再灌注大鼠全血碳氧血红蛋白(COHb)含量、脑组织血红素氧化酶1(HO1)活性的变化及左旋四氢巴马汀(L THP)对其的影响。方法:77只SD大鼠随机分为假手术组(S组,n=7)、脑缺血/再灌注组(I组,n=35)及L THP治疗组(T组,n=35)。I组和T组建立大鼠全脑缺血/再灌注模型,分别于脑缺血/再灌注1、3、12、24和48h各时间点检测脑组织匀浆HO1活性、环磷酸鸟苷(cGMP)及血中COHb含量,并与S组比较。结果:1I组在脑缺血/再灌注后HO1活性、COHb含量及cGMP水平均明显高于S组(P均<0.01)。2T组的HO1活性及COHb在脑缺血/再灌注1、12、24和48h时均显著低于I组(P均<0.01),3h时略低于I组,差异无显著性;HO1活性在各时间点均高于S组(P均<0.01);COHb含量在24h和48h时略低于S组,但差异无显著性,其余各时间点均高于S组(P均<0.01);cGMP水平均显著低于I组(P均<0.01),但高于S组(P<0.01)。3苏木素伊红(HE)染色I组海马CA1区存活神经元数目在脑缺血/再灌注3h后显著减少(P<0.01),应用L THP后存活神经元数目明显高于I组(P<0.01)。结论:L THP可通过抑制HO1活性使一氧化碳(CO)和cGMP水平下降,减少神经元丢失,提高存活神经元数目,减轻脑缺血/再灌注损伤。Objective： To observe the changes of hemeoxygenase - 1 （HO - 1） activity in cerebral tissue, carboxyhemoglobin （COHb） level in blood of rats during global cerebral ischemia/reperfusion （I/R） and the influence of L - tetrahydropalmatine （L - THP） on them. Methods ： Seventy-seven Sprague-Dawley （SD） rats were randomly divided into sham -operated group （S group, n=7）, I/R group （I group, n=35） and L -THP treatment group （T group, n=35）. Cerebral I/R model was reproduced in SD rats of I group and T group. HO -1 activity, cyclic GMP （cGMP） in the brain and COHb in blood were evaluated respectively at 1, 3, 12, 24, and 48 hours after global cerebral I/R and compared to those of the S group. Results： （1） HO 1 activity, COHb content and cGMP level in I group increased as compared with those in S group after global cerebral I/R （all P〈0.01）. ② HO -1 activity and COHb content of T group were significantly lower than those of I group at 1, 12, 24, and 48 hours after cerebral I/R （all P〈0.01）, at 3 hours they were slightly lower than those of 1 group, the difference being not significant; HO - 1 activities at all time points were higher than those of S group （all P〈0.01） ; COHb contents at 24, 48 hours were slightly lower than those of S group, the difference being not significant, and at all the other time points the COHb contents were higher than those of S group （all P〈0.01）. eGMP level of T group was significantly lower than that of I group at all time points （all P〈0.01）, but higher than those of S group （all P〈0.01 ）. ③Hematoxylin and eosin （HE） stain showed that the number of surviving neurons in hippocampus CA1 region of I group was decreased at 3, 12, 24, and 48 hours after cerebral I/R （all P〈0.01）, but there was no significant change at 1 hour. The number of surviving neurons in T group was much higher than that in I group （P〈0. 01）. Conclusion; L THP could decrease carbon monoxide content and cGMP level by inhibi

关 键 词：血红素氧化酶 一氧化碳 缺血/再灌注损伤 脑 左旋四氢巴马汀 

分 类 号：R743[医药卫生—神经病学与精神病学]