不同深度牙体窝洞制备对人成牙本质细胞影响的实验研究  

Influence of different depth in cavity preparations on adult odontoblasts

作　　者：王晓春[1,2] 梁景平[1,2] 陈颖[1,2] 郭炳诗 张秀丽[4] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔内科 [2]上海市口腔医学研究所 [3]中国科学院上海生命科学研究院,上海交通大学医学院健康科学研究所 [4]上海市口腔医学研究所,上海市口腔医学重点实验室,上海200011

出　　处：《牙体牙髓牙周病学杂志》2006年第4期195-197,共3页Chinese Journal of Conservative Dentistry

摘　　要：目的：建立牙体制洞的体外实验模型，研究不同深度的窝洞对人成牙本质细胞的影响。方法：20～30岁成人健康第三磨牙40个，随机分为4组（3个实验组和1个对照组），分别制浅（A组）、中（B组）、深（C组）3种不同深度窝洞，冠根分开，37℃，50mL／LCO2连续培养2d，每天换液。扫描电镜检测成牙本质细胞形态，显微图像分析系统计算台盼蓝染色率，并进行统计学分析。结果：髓室顶中央区域，牙本质小管开放，仅见极少量的细胞碎片，周围区域成牙本质细胞形态良好；各实验组之间的台盼蓝染色率在统计学上有显著性差异（P〈0．01）。结论：制备窝洞不同深度均对成牙本质细胞造成损伤，随着制洞深度的增加，成牙本质细胞受到的损伤明显加重。AIM：To establish a model for cavity preparations in situ and to study the influence of different depth on adult odontoblasts. METHODS： Forty healthy third molars obtained from 20 - 30 year old individuals were randomly divided into one control and three experiment groups. For the latter, shallow （group A ）, medium （group B ）, deep（ group C ）cavity preparations were performed separately. The root was dissected from the crown, and odontoblasts in the crown were cultured for up to 2 days at 37℃ ,50 mL/L CO2 ,and the medium was replaced daily. Cell morphology was analyzed by scanning electron microscope, and cell vitality was determined by trypan blue staining and accorded by a micro image analysis system. RESULTS：In the central region of the pulp roof, the dentin tubes were open and only few cells fragments remained. In the peripheral region of the pulp roof, the odontoblast maintained good cell morphology. In addition, there was a significant difference in the trypan blue staining rate among the three experimental groups （P 〈0.01 ）. CONCLUSION：The odontoblasts were damaged by different depth cavity preparations, cell damage became more aggravated,the deeper the cavity preparations.

关 键 词：成牙本质细胞 制洞 显微图像分析 

分 类 号：R781.05[医药卫生—口腔医学]