整合素连接激酶mRNA在非小细胞肺癌组织中的表达和意义  

The expression of integrin-linked kinase (ILK) mRNA and the clinical significance in non-small cell lung cancer

作　　者：邬麟[1] 陈琼[2] 杨一新[3] 胡成平[2] 杨红忠[2] 

机构地区：[1]湖南省肿瘤医院内科,长沙410013 [2]中南大学湘雅医院呼吸内科,410008 [3]中南大学湘雅医学院肿瘤研究所,410008

出　　处：《临床肿瘤学杂志》2006年第1期23-26,共4页Chinese Clinical Oncology

摘　　要：目的:比较整合素连接激酶(ILK)mRNA在非小细胞肺癌(NSCLC)组织及正常肺组织的表达差异,分析ILKmRNA的表达与NSCLC的恶性表型、侵袭程度、淋巴转移、远处转移、临床分期之间的关系。方法:取NSCLC患者及肺部良性病变手术切除标本,液氮及超低温冰箱保存,TRIzol法提取总RNA,RTPCR扩增ILKmRNA后对扩增产物进行电泳及图像分析。结果:35例肺癌、30例癌旁组织、29例正常肺及10例肺部良性病变组织ILKmRNA定性表达阳性率分别为82.9%(29/35)、76.7%(23/30)、55.2%(16/29)和60%(6/10),癌组织与正常肺组织之间ILKmRNA阳性率比较有显著性差异(P=0.027)。ILKmRNA定量表达为肺癌组1.67±0.349,癌旁组0.86±0.185,正常组0.43±0.102,肺部良性病变组0.78±0.201,除癌旁组织和肺部良性病变组织差别无统计学意义外,其余差异均有显著性(P<0.01)。NSCLC组织ILKmRNA定量表达的均值随细胞恶性程度、淋巴转移和远处转移及临床分期递增而随之递增(P<0.05)。结论:ILKmRNA在NSCLC组织中表达量高于癌旁组织、正常肺及肺部良性病变组织。高表达的ILKmRNA预示着NSCLC的高度恶性、高侵袭性、高转移性和预后不良,ILKmRNA可作为反映NSCLC恶性表型、转移和侵袭特性的分子标记物。Objective：To investigate the difference of integrin-linked kinase （ILK） expression in NSCLC tissue and normal lung tissue and analyze the relationship among the ILK expression and malignant type, invasive degree, lymph node metastasis, distant metastasis and clinical stage in NSCLC. Methods：The operative sample of NSCLC and benign lung lesion tissue were studied. Total RNA was extract with TRIzol. The expression of ILK mRNA was detected by reverse-transcription polymerase chain reaction （ RT- PCR）, followed by electrophoresis and image analysis. Results：The ILK mRNA expression positive rate in 35 cases lung carcinoma, 30 cases adjacent, 29 cases non-neoplastic normal lung tissue and 10 cases benign lung lesion were 82. 9% （23/35）, 76.7% （ 23/30）, 55.2% （ 16/29 ） and 60% （6/10） respectively. The difference between lung carcinoma and normal lung tissue had statistical significance （ P = 0. 027 ）. The quantitative expression in lung carcinoma, adjacent and non-neoplastic normal lung tissue and benign lung lesion were 1.67 ±0. 349, 0. 86 ±0. 185, 0. 43 ±0. 102 and 0. 78 ±0. 201 respectively. The difference between each other had statistical significance （P 〈0.01 ） except adjacent and benign lung lesion. The quantitative expression of ILK mRNA was strongly correlated with malignant type, lymph node metastasis, distant metastasis and clinical stage in NSCLC （P 〈 0.05 ）. Conclusion：The expression of ILK mRNA is higher in NSCLC than in adjacent, normal lung and benign lung lesion tissue. Strong expression of ILK mRNA indicates higher malignancy, invasion, metastasis and lower survival of NSCLC. ILK mRNA might be a novel molecular marker for aggressive NSCLC.

关 键 词：整合素连接激酶 肺癌 

分 类 号：R730.43[医药卫生—肿瘤] R734.2[医药卫生—临床医学]