Insecticidal activity of residual Bt protein at the second trophic level  

作　　者：SHI Hong ZHANG Lifu HUA Baozhen MI Xiangcheng WEI Wei ZHANG Yongjun MA Keping 

机构地区：[1]Laboratory of Quantitative Vegetation Ecology, Institute of Botany,Chinese Academy of Sciences, Beijing 100093, China [2]College of Plaint Protection, North-west Agriculture and Forestry University, Shaanxi 712100, China [3]College of Agriculture and Forestry, Linyi Normal University, Shandong 276005, China [4]Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China

出　　处：《Chinese Science Bulletin》2006年第8期946-951,共6页

基　　金：supported by part of a 973 Project(Grant No.2001CB109004);National Natural Science Foundation of China(Grant No.30270228);a grant of the President Foundation of CAS,China,to Wei Wei.

摘　　要：Measurements were taken of Bt protein expressed in the leaves of transgenic cotton (Gos- sypium hirsutum) transformed with a synthesized Bt (Bacillus thuringiensis) cry1A gene and its persistent level in larval bodies and faeces of a non-targeted insect pest, beet armyworm (Spodoptera exigua). We performed enzyme linked immunosorbent assays (ELISA) and bioassays using neonate larvae of cot- ton bollworm (Helicoverpa armigera) to detect the insecticidal activity of residual Bt protein at the sec- ond trophic level. The results showed that Bt protein content in functional leaves was different at various developmental stages and was different among plants at the same stage. Even though Bt protein concentration in the larval bodies and faeces de- creased 97.5%―99% compared to that found in cotton leaves subsequently fed to beet armyworm larvae, it still had a lethal effect on neonate cotton bollworm larvae. Therefore, Bt protein present at the second trophic level had insecticidal activity. This result is important in understanding and predicting the effect of transgenic plants on nontarget organisms.Measurements were taken of Bt protein expressed in the leaves of transgenic cotton （Gossypium hirsutum） transformed with a synthesized Bt （Bacillus thuringiensis） cry1A gene and its persistent level in larval bodies and faeces of a non-targeted insect pest, beet armyworm （Spodoptera exigua）. We performed enzyme linked immunosorbent assays （ELISA） and bioassays using neonate larvae of cotton bollworm （Helicoverpa armigera） to detect the insecticidal activity of residual Bt protein at the second trophic level. The results showed that Bt protein content in functional leaves was different at various developmental stages and was different among plants at the same stage. Even though Bt protein concentration in the larval bodies and faeces decreased 97.5%-99% compared to that found in cotton leaves subsequently fed to beet armyworm larvae, it still had a lethal effect on neonate cotton bollworm larvae. Therefore, Bt protein present at the second trophic level had insecticidal activity. This result is important in understanding and predicting the effect of transgenic plants on nontarget organisms.

关 键 词：萃取溶液 幼虫 粪便 风险评估 杀虫剂 蓝四氮唑 蛋白质 

分 类 号：S482.3[农业科学—农药学]