马铃薯新型栽培种后代低还原糖材料的筛选与评价  

Evaluation and Selection of Low Reducing Sugar Clones in the Progenies of Neo-tuberosum

作　　者：张庆娜[1] 张立波 石瑛[1] 朱海霞[1] 张铁强[1] 陈伊里[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]北京大兴旧宫中学,北京100076

出　　处：《中国马铃薯》2006年第2期73-77,共5页Chinese Potato Journal

基　　金：国家"863"计划项目(2004AA241133)资助

摘　　要：以新型栽培种6个杂交组合后代的156份无性系为供试材料,利用尿糖试纸法进行低还原糖材料的初筛选后,再对低还原糖材料进行低温贮藏和回暖后还原糖、干物质含量的精确测定。试验结果表明:利用尿糖试纸法筛选出19份显色为1级的材料,采用3,5-二硝基水杨酸比色法精确测定,还原糖含量小于0.4%的有16份,证明应用此法进行马铃薯块茎还原糖含量的初筛选是有效的。低温贮藏和回暖试验结果显示,无性系1、2、4和7为耐低温糖化类型,无性系3、5、6、8、9、12、13和15属于回暖反应明显的类型,无性系10、11、14和16属于低温糖化明显的类型。In this research, 156 clones from 6 crosses with Neo-tuberosum in their background were screened for low tuber reducing sugar content using uric sugar test paper. The clones selected were re-tested for low reducing sugar content by the colorimetry method （3,5-dinitrosalicylic acid）. Then, the clones were monitored for the reducing sugar and dry matter contents, of tubers after low temperature storage and recorid.itioning. Out of 19 clones selected by uric sugar test paper, 16 clones were lower in reducing sugar content than 0.4% when tested by the colorimetry method, suggesting that uric sugar test paper can be effective for screening preliminarily for low reducing sugar in a segregating population. The 16 clones were grouped into 3 types according to their response to low temperature storage and reconditioning：.the clones 1, 2, 4 and 7, resistance to cold-induced sweetening;clones 3, 5, 6, 8, 9, 12, 13, and 15, susceptible to cold-induced sweetening, but responding well to reconditioning; and the clones 10, 11, 14, and 16, susceptible to cold-inducing sweetening and not responding well to reconditioning.

关 键 词：马铃薯 还原糖 干物质 尿糖试纸 回暖 

分 类 号：S532[农业科学—作物学]