南极酵母Rhodotorula sp.NJ-298产虾青素的初步研究  

Study on antarctic yeast Rhodotorula sp.NJ-298 producing astaxanthin

作　　者：刘均玲[1,2] 缪锦来[3] 王全富[1] 郑洲[3] 李光友[3] 

机构地区：[1]中国海洋大学生命学院 [2]国家海洋局海洋生物活性物质重点实验室,山东青岛266061 [3]国家海洋局海洋生物活性物质重点实验室

出　　处：《中国海洋药物》2006年第2期17-21,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金资助项目(40406003)

摘　　要：一株从南极海冰中分离的酵母菌NJ-298可产生类胡萝卜素,总类胡萝卜素产量为1986μg.g-1。紫外可见分光光度计扫描,LC/MS分析表明虾青素为其主要成分,HPLC分析测定其含量为总色素的81.9%。对南极酵母NJ-298生长研究表明虾青素的合成主要集中在对数生长期和稳定期,最大产量为6506μg.L-1。优化培养结果表明NJ-298最适生长温度为8℃,醋酸钠和蛋白胨为最适碳氮源。并且该色素具有较强的清除自由基DPPH能力,IC50为0.40μg.mL-1。本研究表明,利用南极酵母Rhodotorulasp.NJ-298是获取虾青素的新途径。A yeast strain Rhodotorula sp. NJ-298, which was isolated from antarctic sea-ice, could produce carotenoids pigment,and the total production was 1986μg·g^-1. UV-visible spectrum and LC/MS analysis indicated astaxanthin was the major component of the carotenoids, and HPLC analysis showed its content accounted for 81.9 % of total carotenoids productoin. Maximum astaxanthin production 6506 μg·L^-1 was obtained when the yeast was grown at steady-state phase. Optimum temperature for the strain grow was at 8℃, and sodium acetate and peptone were the best carbon and nitrogen sources for the astaxanthin production. Moreover, the pigment had strong activity of scavenging free radicals, and the IC50 was 0.40 μg·mL^-1 by DPPH assay.

关 键 词：南极酵母 RHODOTORULA sp. 类胡萝卜素 虾青素 自由基 

分 类 号：Q936[生物学—微生物学]