人参皂苷Rb_3对照品的制备研究  

Studies on Preparation of Standard Substance Ginsenoside Rb_3

作　　者：饶高雄[1] 张维明[1] 王梅[2] 范亚刚[2] 陈纪军[3] 

机构地区：[1]云南中医学院中药学院,云南昆明650200 [2]云南省药品检验所,云南昆明650011 [3]中国科学院昆明植物研究所,云南昆明650204

出　　处：《云南中医学院学报》2006年第2期8-10,共3页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南省中青年学术和技术带头人培养基金

摘　　要：目的:以三七叶为原料,研究人参皂苷Rb3对照品的制备与分析技术。方法:通过色谱方法分离三七叶中人参皂苷Rb3,用光谱方法鉴定结构,并用TLC、HPLC方法检查纯度和标定含量。结果:人参皂苷Rb3对照品经TLC检查无杂质斑点,二维及三维HPLC检查为单一成分,峰纯度因数999.60,含量为99.84%.结论:人参皂苷Rb3对照品达到中药质量标准用化学对照品技术要求,已列入《中国药典》2005年版。Objective ：To study the preparation and analysis technique of standard substance ginsenoside Rb3. Methods： The ginsenoside Rb3 was isolated from Panax leave by chromatographer, and its structure was identified by spectrum analysis. The TLC and HPLC were employed for inspection its purity and content.Results： The TLC showed the ginsennoside Rb3 has no impurity spot. Two-dimension and Three-dimension HPLC indicated that it＇s a single peak with the purity factor 999.60,and its content was 99.84%. Conclusion： The ginsenoside Rb3 has been gathered as a standard substance by the Chinese pharmacopoeia 2005 edition.

关 键 词：人参皂苷RB3 对照品 制备方法 三七叶 七叶神安片 

分 类 号：R284.2[医药卫生—中药学]