PDGF及其受体在大鼠胰腺纤维化中的作用  

Expression of PDGF and its receptor on pancreas fibrosis in rats

作　　者：王东盛[1] 刘倩[2] 寇妍[3] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东省千佛山医院消化科,山东济南250014 [3]济南第四人民医院烧伤科,山东济南250031

出　　处：《山东大学学报（医学版）》2006年第4期360-363,共4页Journal of Shandong University:Health Sciences

摘　　要：目的:探讨血小板源生长因子(PDGF)及其受体(PDGFR)在胰腺纤维化过程中的作用及意义。方法:通过胰管内注射2%三硝基苯磺酸(TNBS)的方法制作大鼠胰腺纤维化模型,采用免疫组化方法检测2、3、45、6、周PDGF、PDGFR及α-平滑肌肌动蛋白(-αSMA)在大鼠胰腺组织中的表达。结果:第4周,PDGF-B、PDGFR-β、-αSMA在模型组的表达均强于对照组(P均<0.01)。-αSMA与PDGF-B、PDGFR-β定量分析呈正相关(P均<0.01),与PDGF-A、PDGFR-α无相关关系(P均>0.05)。模型组中不同时期PDGF-B及PDGFR-β表达分别强于PDGF-A、PDGFR-α(P均<0.01)。结论:PDGF、PDGFR在慢性胰腺炎发生纤维化过程中具有重要作用,其中以PDGF-B及PDGFR-β为主。Objective： To study the expression and significance of PDGF and its receptor in the process of pancreas fibrosis. Methods： The animal model of chronic pancreatitis was established by injection of 2% TNBS to rat＇s pancreatic duct. Expressions of PDGF, PDGFR and α-SMA were determined in pancreas tissues in different stages by using immnnohistochemistry technique. Results： Expressions of PDGF-B, PDGFR-β and α-SMA were stronger in model than in control group on the fourth week. There was a positive correlation between α-SMA and PDGF-B and PDGFR-β, and no correlation between α-SMA and PDGF-A and PDGFR-α. The expressions of PDGF-B and PDGFR-β were stronger than those of PDGF-A and PDGFR-α with a significant difference in model group. Conclusion： PDGF-B and PDGFR-β are mainly to act play a key role in pancreas fibrosis and development.

关 键 词：受体 血小板源生长因子 胰腺炎 大鼠 Wister 

分 类 号：R576[医药卫生—消化系统] R-332[医药卫生—内科学]