雄黄治疗胶质瘤的初步试验研究  

The pilot study on realgar for glioma

作　　者：庞琦[1] 王汉斌[1] 葛明旭[1] 司志超[1] 朱玉方[1] 张康[1] 魏广福[1] 张志明[2] 

机构地区：[1]山东大学山东省立医院神经外科,山东济南250021 [2]美国肯塔基州立大学医学院

出　　处：《山东大学学报（医学版）》2006年第4期376-379,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2005C31)

摘　　要：目的:研究雄黄体内外对胶质瘤细胞的抗肿瘤作用,评价雄黄作为一种新的抗肿瘤药物应用于治疗胶质瘤的可行性。方法:采用体外细胞增殖实验和细胞形态学变化研究雄黄体外对胶质瘤细胞的生长抑制作用;大鼠皮下接种胶质瘤细胞,建立胶质瘤动物模型,用局部注射的方法治疗肿瘤,观察雄黄体内抗肿瘤的作用。结果:雄黄对体外培养的胶质瘤细胞9 L有明显的生长抑制作用,4 h1、2 h和24 h的抑制率分别为40%、78%和99%;体内抑瘤实验显示,局部用药3周后,75%(9/12)的大鼠肿瘤消失,3个月内的复发率为8.3%。结论:雄黄在体内外对胶质瘤均有较强的抗肿瘤作用,有可能用于胶质瘤的临床治疗。Objective： To investigate the potential effects of realgar on the glioma cells both in vitro and in vivo, and evaluate its role as a novel agent against human glioma. Methods： The 9 L glioma cells were cultured in suitable medium, cell proliferation inhibitory assay was employed to observe the reaction of these cells, including the morphological changes and cytotoxicity by realgar suspension at a certain concentration; local injection or oral admin- istration or both of them were applied on the established subcutaneous 9 L glioma animal models and the corresponding reaction was observed. Results： Realgar showed strong inhibitory effects on the 9 L glioma cells in vitro. According to the trypan blue coloration and cell counting, the suppression ratio of the cells on 4 h, 12 h and 24 h was 40%, 78% and 99% respectively; in the animal model experiment, strong anti-tumor effects were also observed. In the local injection group, about 75% of the tumours disappeared in less than 3 weeks after injection, and about 8.3% of the tumours relapsed in 3 months. Condusion： Realgar has strong anti-tumor effects both in vitro and in vivo,and it is a potential anti-glioma agent.

关 键 词：雄黄 砷剂 神经胶质瘤 9L细胞 大鼠 

分 类 号：R739.41[医药卫生—肿瘤] R-332[医药卫生—临床医学]