温肾方对实验性自身免疫性甲状腺炎大鼠TNF-α的影响  

Eefect of wenshenfang on the relative expression of TNF-α in expermiental autoimmune thyroiditis

作　　者：顾志峰[1] 余江毅[2] 秦文浩[1] 崔世维[1] 曹晓蕾[3] 

机构地区：[1]南通大学附属医院内分泌科,江苏226001 [2]江苏省中医院内分泌科 [3]南通大学基础医学院

出　　处：《中国交通医学杂志》2006年第2期157-158,共2页Chinese Medical JOurnal of Communications

基　　金：江苏省科技厅;省333人才资助课题(No.BK2002135)。

摘　　要：目的:研究温肾方对实验性自身免疫性甲状腺炎(EAT)大鼠肿瘤坏死因子α影响。方法:通过RT-PCR方法对SD大鼠EAT甲状腺组织TNF-α的测定,了解温肾方等对EAT发生的干预机制。结果:EAT大鼠的TNF-α水平均明显高于温肾方组、雷公藤组、对照组(P<0.05)。结论:温肾方可能通过对TNF-α的抑制而对EAT能起到有效的预防及治疗作用。Objective：To investigate the effect of Wenshenfang in the treatment of experimental antoimmune thyroiditis （EAT） by observing the level of tumor necrosis faetor-αlpha（TNF-α） in thyroid tissues. Methods：An EAT model was established in SD rats by injection of swine thyroglobin combined with adjuvant. Its mechanism was studied by detecting the relative expressions of TNF-α. Resuit： The relative expressions of TNF-α were fewer in normal control and the rats treated with Tripterygium Wilfordii Hook for Wen- Shenfang than in EAT rats without treatment （P〈0.05）. Conclusion： Wenshenfang has a therapeutic effect on EAT. The mechanism by which Wenshenfang exerts its effects may be by the suppression of relative expressions of TNF-α in thyroid cells.

关 键 词：实验性自身免疫性甲状腺炎 细胞因子 肿瘤坏死因子Α 温肾方 

分 类 号：R259.814[医药卫生—中西医结合]