微生物催化D-葡萄糖与酪醇葡糖基转移合成红景天甙的初步研究  

Preliminary Study on Synthesis of Salidroside through Glucosylation of D-Glucose and Tyrosol Catalyzed by Microorganism

作　　者：王梦亮[1] 张芳[1] 刘滇生[1] 

机构地区：[1]山西大学现代化学研究所,山西太原030006

出　　处：《催化学报》2006年第3期233-236,共4页

基　　金：山西省科委攻关项目(051039-4)

摘　　要：为了探讨微生物催化合成红景天甙的可能性,从红景天根系土壤中筛选出了五个菌株,比较了它们合成红景天甙的能力,确定F1菌株为合成红景天甙的出发菌株,经鉴定该菌株为米曲霉(Aspergillus oryzae).以D-葡萄糖和酪醇为底物,研究了底物浓度、反应时间、细胞浓度和pH对红景天甙产量的影响.结果表明,最适底物浓度为酪醇5 g/L,D-葡萄糖与酪醇的摩尔比为1∶1,酪醇浓度高于5 g/L不利于菌体生长.最佳反应时间为48 h,最适细胞浓度为150 g/L,最适pH为7.红景天甙最高产量为0.7 g/L.Salidroside is an important pharmacologically active compound in the traditional Chinese medicinal herb Rhodiola rosea L. In this work, five strains were isolated from soil around the roots of herb, and their catalytic activity for the synthesis of salidroside from D-glucose and tyrosol was tested. F1 strain, which was identified as Aspergillus oryzae, produces the highest amount of salidroside among the five strains. Therefore, its whole cells were used as catalyst for the synthesis of salidroside from D-glucose and tyrosol. The content of salidroside and tyrosol was analyzed by high-performance liquid chromatography. The results show that the highest yield of salidroside is 0.7 g/L under the optimal conditions, i.e. , the tyrosol concentration of 5 g/L, reaction time of 48 h, cell concentration of 150 g/L, 30 ℃, and pH of 7. When the tyrosol concentration in the medium is above 5 g/L, the cell growth and the salidroside synthesis are both inhibited. Salidroside is only found inside the cells.

关 键 词：红景天甙 微生物 米曲霉 生物催化 酪醇 葡萄糖 葡糖基转移 

分 类 号：TQ464[化学工程—制药化工]