急性白血病中c-kit受体表达与染色体异常核型的关系  

Relationship between expression of c-kit receptor and abnormal chromosome in acute leukemia

作　　者：时昊[1] 朱锋[1] 肖爱芹[1] 张志瑢[1] 张日[2] 

机构地区：[1]山东泰山医学院附属医院血液科,山东泰安271000 [2]江苏苏州大学附属第一医院血液科,江苏苏州215006

出　　处：《中国现代医学杂志》2006年第8期1157-1159,1163,共4页China Journal of Modern Medicine

基　　金：山东省教育厅科技计划项目(J05L09)

摘　　要：目的检测c-kitR在急性白血病(acuteleukemia,AL)的表达并进一步分析ANLL中c-kitR表达与染色体异常核型的关系及其意义。方法采用流式细胞术(flowcytometry,FCM)分别检测并比较92例急性淋巴细胞性白血病(ALL)和81例ANLL初诊患者骨髓单个核细胞(BMMNC)跨膜c-kitR表达的阳性率及阳性水平,并设立20例正常人骨髓标本作为阴性对照组;采用R带显带方法对c-kitR阳性表达的ANLL进行染色体核型分析。结果ALL患者的c-kitR表达的阳性率及阳性水平与正常人相比差异无显著性(P>0.05)。ANLL患者的c-kitR表达率及阳性水平则均明显高于正常人和ALL患者(P值分别<0.01)。ANLL中c-kitR阳性表达者异常核型的百分率为74.1%,明显高于c-kitR阴性组者(26.1%)(P<0.05)。异常核型的类型有5q-、-17、+21、del3p、t(8;21)、6p+、t(1;8)、复杂易位、t(15;17)、t(1;12)、-7、四倍体、20q-等十余种类型。结论c-kitR可作为髓系免疫学标记,用以协助ANLL的诊断。c-kitR阳性者伴有细胞遗传学异常,但是,c-kitR阳性与染色体异常核型的类型之间不存在特殊对应关系。c-kit原癌基因不是ANLL发病机制的唯一要素。[Objective] To explore the expression of c-kit receptor （c-kit R, CD117） in acute leukemia （AL）. The focus of the study was laid on relationship between expression of c-kit R and abnormal karyotype in ANLL, [Methods] Flow cytometry （FCM） was used to determine the expression of the transmembrane c-kit R on the bone marrow mononuclear cells （BMMNC） in 92 patients of ALL and 81 patients of ANLL. Chromosome R banding technique was used to assay the karyotype in ANLL with positive expression of c-kit R. [Results] There was no significant difference between expression of c-kit in health individuals and patients of ALL（P 〉0.05）. The rate of c-kit R expression in patients of ANLL was significantly higher than that in healthy individuals and that in patients of ALL as well （P 〈 0.01 respectively）. Percentage of abnormal karyotype in the group of positive expression of c-kit R is 74,1%, which was significantly higher than that of 26.1% in the group of negative expression of c-kit R （P 〈0.05）. There were many types of abnormal karyotype in ANLL with positive expression of c-kit R. [Conclusion] c-kit R can be used as the immunology indicator for diagnosis of ANLL. Expression of c-kit R is correlated with abnormal chromosome. But there is no any specific corresponding relationship between the c-kit R expression and the types of abnormal karyotype in ANLL. c-kit oncogene will not be sole premise of pathogenesis of ANLL.

关 键 词：白血病 急性 C-KIT受体 染色体 白血病 非淋巴细胞 急性 

分 类 号：R733.71[医药卫生—肿瘤]