小鼠白介素-12在人卵巢癌SKOV_3中的抗肿瘤作用  

Transfected miL-12 genes against ovarian cancers

作　　者：刘娟[1] 王晶[1] 隋丽华[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院妇科,哈尔滨黑龙江150040

出　　处：《中国现代医学杂志》2006年第8期1171-1173,1176,共4页China Journal of Modern Medicine

基　　金：黑龙江省攻关课题(No:GC04C31003)

摘　　要：目的观察转染至SKOV3卵巢癌细胞中小鼠IL-12全长基因的质粒能稳定表达相关细胞因子基础上,在脾细胞存在的情况下对SKOV3肿瘤细胞的杀伤作用。方法MTT方法检测对SKOV3卵巢癌细胞增殖的影响,免疫组化法检测SKOV3卵巢癌细胞表面IL-12蛋白的表达。结果转染后癌细胞免疫组化可见IL-12表达,在脾细胞的作用下,MTT结果显示IL-12对SKOV3卵巢癌细胞有抑制其增殖的作用,P<0.05。结论成功地将IL-12基因转染至SKOV3卵巢癌细胞中并稳定表达相关细胞因子,在脾细胞存在情况下对SKOV3细胞有抑制其增殖作用,从而杀伤卵巢癌细胞,具有抗肿瘤作用。[Objective] To investigate Interleukin-12 genes anti SKOV3 ovarian cancer cell effects along with mouse spleen cells. [Methods] Following to culture 1×10^6 spleen cell with suspension and transfected tumor ceils, proliferation effects was evaluated by MTT. [Results] SKOV3 ovarian cancer cells＇ proliferation were restrained by IL-12 after co-cultured with spleen cells, P 〈0.05. We found the expression of IL-12 from ceils surface, in contrast, negative results of ovarian cancer ceils without transfected with immunochemistry. [Conclusion] With mice spleen ceils, transfected SKOV3 ovarian cancer ceils can express IL-12 and kill ovarian cancer ceils by eliciting NK T ceils to produce such cytokines as INF-γ.

关 键 词：白细胞介素-12 卵巢癌 基因治疗 

分 类 号：R737.31[医药卫生—肿瘤]