顶体形态与精子活力、活率关系分析  

Correlation between acrosomal morphology and sperm motility,viability

作　　者：王瑞雪[1] 刘睿智[1] 许宗革[1] 薛百功[1] 

机构地区：[1]吉林大学基础医学院细胞生物学教研室,吉林长春130021

出　　处：《中国现代医学杂志》2006年第8期1210-1212,共3页China Journal of Modern Medicine

基　　金：吉林省科技厅资助项目(20030433)

摘　　要：目的探讨精子顶体形态与精子活力、活率关系。方法123例男性不育患者,采用精子自动分析质量检测系统检测精液常规,采用伊红Y水实验分析精子活率,采用联苯胺染色计数精液白细胞浓度,用改良巴氏染色法分析精子形态及顶体形态。结果精子活率正常组顶体完整率明显高于精子活率异常组(P<0.05);精子活力正常组与精子活力异常组顶体完整率比较差异无显著性(P>0.05);精液参数正常组顶体完整率明显高于精液参数异常组(P<0.05);顶体完整率与正常形态精子百分率、精子活率呈显著正相关关系(P<0.05,P<0.01),与白细胞浓度呈显著负相关关系(P<0.05)。结论顶体形态分析可以在一定程度上反映男性生育力,是男性不育症诊断的一个有价值指标。[Objective] To investigate the relationship between sperm acrosomal morphology and sperm motility, viability. [Methods] Human semen samples were collected from 123 male patients with infertility. Semen parameters were assessed by using CASA. Sperm viability and semen leukocytospermia concentration were detected respectively by means of eosin Y stain and benzidine stain. Sperm morphology and acrosomal morphology were evaluated after modified papanicolaou stain. [Results] The rate of intact acrosome in normal sperm viability group was significandy higher than that in abnormal sperm viability group （P 〈0.05）. No significant differences were observed in the comparison between normal sperm motility group and abnormal sperm motility group （P 〉0.05）. Compared with abnormal semen parameters group, the rate of intact acrosome was increased in normal semen parameters group （P 〈 0.05）. The rate of intact acrosome was positively correlated with the percentage of morphologically normal sperm and sperm viability （P 〈0.05, P 〈0.01）, and negatively correlated with the semen leukocytospermia concentration （P 〈 0.05）. [Conclusion] Sperm acrosomal morphology can reflect the potential of male fertility to some extent and can be regarded as a good predictor for diagnosis of male infertility.

关 键 词：精子顶体 精子活力 精子活率 精子形态 

分 类 号：R394[医药卫生—医学遗传学]