铬鞣革屑固定化1398中性蛋白酶的制备和应用  

Preparation of Immobilization 1398 Neutral Protease by Chrome Tanned Shavings

作　　者：马兴元[1] 张韬琳[1] 俞从正[1] 董新宽[1] 陈静[1] 

机构地区：[1]陕西科技大学资源与环境学院,陕西咸阳712081

出　　处：《皮革科学与工程》2006年第2期58-61,共4页Leather Science and Engineering

基　　金：陕西科技大学校自然科学基金(ZX05-12);咸阳市科技局项目(K0410-1)基金;北京泛博基金

摘　　要：以铬鞣革屑为载体、戊二醛为交联剂,探讨了影响1398中性蛋白酶固定的因素。确定了制备固定化1398中性蛋白酶的最佳条件:温度35℃,pH值7.0,戊二醛与载体反应的时间4 h,酶与载体反应的时间8 h,戊二醛用量0.11%,酶用量3.1%。用铬鞣革屑固定化1398蛋白酶催化水解明胶,采用串连柱型反应器,可方便的控制水解明胶相对分子质量的大小。1398 neutral protease was immobilized by chrome tanned shavings with glutaraldehyde as cross-linking agent. The effects of temperature, pH, amount of cross-linking agent and ratio of enzyme to carrier on the activity of the immobilized enzyme were demonstrated, and the optimum immobilization condition of 1398 neutral protease was established. The glutin has been hydrolyzed by immobilization 1398 neutral protease, can used series-wound pillar reactor, can control molecular of hydrolyzed glutin.

关 键 词：固定化酶 铬鞣革屑 蛋白酶 戊二醛 

分 类 号：TS529.1[轻工技术与工程—皮革化学与工程]