特定分子量范围内香菇多糖的分离研究  

Study on the Isolation of Lentinan within Definite Molecular Weights

作　　者：张红旭[1] 郭辉[1] 

机构地区：[1]江苏徐州市第三人民医院,徐州市221005

出　　处：《中国药房》2006年第8期575-576,共2页China Pharmacy

摘　　要：目的:研究平均分子量为400000～600000范围内香菇多糖的分离工艺。方法:将精制的香菇多糖进行2—二乙氨基乙基—纤维素阴离子交换柱色谱纯化,再通过葡聚糖凝胶G-200柱层析法进行分离;以高效凝胶色谱法测定香菇多糖的分子量。结果:分离得到的香菇多糖平均分子量为400000～600000。结论:本方法可用于香菇多糖的分离。OBJECTIVE： To study the isolation of lentinan, the average molecular weight of which ranged within 400 000 to 600 000. METHODS： The fine lentinan was purified by 2-diethyllaminoethyl-cellulose anion exchange column chromatography, which was then isolated by dextran gel G- 200 column chromatography with the molecular weights determined by high performance gel chromatography. RESULTS： The average molecule weight of the isolated lentinan ranged from 400 000 to 600 000. CONCLUSION： This method can be used for the isolation of lentinan.

关 键 词：香菇多糖 分子量 分离 工艺 

分 类 号：R284.2[医药卫生—中药学]