甘草根瘤菌的16S rDNA全序列测定及系统进化分析  

Determination and Phylogenetic Analysis of Whole 16S rDNA Sequence in Glycyrrhiza Rhizobia

作　　者：杨雪颖[1] 张执欣[1] 杨亚珍[1] 韦革宏[1] 

机构地区：[1]西北农林科技大学生命科学学院微生物研究中心,陕西杨陵712100

出　　处：《西北植物学报》2006年第4期707-711,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30470040)全国优秀博士学位论文作者基金项目(200254)教育部新世纪优秀人才支持计划项目(NCET-04-0953)西北农林科技大学拔尖人才支持计划项目

摘　　要：通过对西北干旱半干旱地区68株甘草根瘤菌的表型多样性和抗逆性分离研究,发现1个新类群和1个具有较高抗逆性的菌株。对其中心菌株CCNWGX022和高抗性菌株CCNWGX035进行16S rDNA全序列测定及系统进化研究。结果表明,CCNWGX022和CCNWGX035与中慢生根瘤菌属内参比菌株的16S rDNA相似性分别大于96．8％和98．3％,因此它们均属于中慢生根瘤菌属。Phenotypes and resistances of 68 strains of Glycyrrhiza rhizobia in aird and semiarid regions of northwest China were separately studied and then a new sub-group and a highly resistant strain were discovered. Whole 16S rDNA sequences of the central strain CCNWGX022 in the subgroup and the highly resistant strain CCNWGX035 were determined and then analyzed in phylogenesis, which indicated that CCNWGX022 and CCNWGX035 were 96.8% and 98.3% similar to the tested strains of Mesorhizobium in 16S rDNA,respectively,and thus both of them belonged to Mesorhizobium.

关 键 词：根瘤菌 甘草 16S rDNA全序列 系统进化 

分 类 号：Q945.13[生物学—植物学] Q939.114