西藏半野生小麦高分子量麦谷蛋白亚基组成分析  

Composition Analysis of High-Molecular-Weight Glutenin Subunits of Semi-natural Wheat (Triticum aestivum ssp.tibetanum Shao) in Tibet

作　　者：彭艳[1] 张金锐[1] 刘勇[1] 何光源[1] 

机构地区：[1]华中科技大学生命科学与技术学院中英HUST-RRes基因工程和基因组学联合实验室,武汉430074

出　　处：《西北植物学报》2006年第4期827-831,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家"863"计划项目资助(2002AA224031)

摘　　要：应用SDS-PAGE分析了50份西藏半野生小麦(Triticum aestivum ssp.tibetanum Shao)的高分子量麦谷蛋白亚基等位基因组成。结果表明,43份材料的HMW-GS组成是同质的,7份材料为异质。供试材料共有7种HMW GS组合,以Null、7+8、2+12为主要类型,占所分析材料的68．4％。在Glu-1位点共检测到10种等位基因,Glu- A1位点2种,Glu～B1位点4种,Glu～D1位点4种。Null(96％)、7+8(80．4％)和2+12(94．9％)分别是Glu-A1、 Glu-B1和Glu～D1位点上主要的等位基因。在Glu-B1位点还新发现2个亚基,暂时分别命名为8*和7**。说明西藏半野生小麦中存在着较广泛的HMW-GS等位基因变异,是小麦品质育种潜在的可利用的遗传资源。The allele compositions of high-molecular-weight glutenin subunits in 50 materials of semi-natural wheat in Tibet were examined by SDS-PAGE. The results showed that 43 of the materials had homogenous HMW-GS compositions and the other seven had heterogeneous HMW-GS compositions. The tested materials totally had seven kinds of HMW-GS compositions of which Null,7＋8 and 2＋12 were the major ones accounting for 68.4%. Ten kinds of alleles were detected at the site Glu-1 ,two kinds of alleles were detected at the site Glu-A1,4 kinds of alleles were detected at the site Glu-B1 and 4 kinds of alleles were detected at the site Glu-D1. Null（96%）,7＋8 （80.4%） and 2＋12（94.9%） were major alleles at Glu-A1, Glu-B1 and Glu-D1 ,respectively. Two new subunits were founded at Glu-B1 and temporarily named 8^＊ and 7^＊＊. These indicated that semi-natural wheat in Tibet contained wide variations of HMW-GS alleles and thus it could be used as the potentially available genetic resources in quality breeding of wheat.

关 键 词：西藏半野生小麦 高分子量麦谷蛋白亚基 等位基因 

分 类 号：Q946.1[生物学—植物学] S512.1[农业科学—作物学]