Interleukin-2 gene-encoded stromal cells inhibit the growth of metastatic cholangiocarcinomas  

作　　者：Myung-HwanKim Sang Soo Lee Sung Koo Lee Seung-Gyu Lee Chul-Won Suh Gyung-Yub Gong Jung-Sun Park Young-Hoon Kim Sang-Hee Kim 

机构地区：[1]Department of Gastroenterology,University of Ulsan College of Medicine,Asan Medical Center,Seoul,Korea [2]Department of Surgery,University of Ulsan College of Medicine,Asan Medical Center,Seoul,Korea [3]Department of Oncology,University of Ulsan College of Medicine,Asan Medical Center,Seoul, Korea [4]Department of Pathology,University of Ulsan College of Medicine,Asan Medical Center,Seoul,Korea [5]Asan Institute for Life Sciences.Seoul,Korea

出　　处：《World Journal of Gastroenterology》2006年第12期1889-1894,共6页世界胃肠病学杂志（英文版）

基　　金：Supported by The Asan Institute for Life Sciences of South Korea,No.2003-013

摘　　要：AIM： To demonstrate bone marrow stromal cells （BMSCs） can be used as an attractive target for genetic modification in the treatment of malignant diseases. METHODS： Using a hamster model of biliary cancer, we investigated the therapeutic effects of interleukin-2 （IL-2） gene-modified BHSCs. Syrian golden hamsters were injected via the femoral vein with 5×10^5 cells of the KIGB-5 biliary cancer cell line （n=20）. One week later, the hamsters were injected intraperitoneally with BMSCs containing Ad/hIL-2 and Ad/△E1, unmodified BHSCs, or RPHI only （control） and observed for 12 wk （n=5/each group）. RESULTS： All hamsters treated with BMSCs containing Ad/hIL-2 survived with no evidence of the disease during this period. In contrast, hamsters in the other three groups showed disseminated metastases involving the lungs as eady as 4 wk.CONCLUSION： Ad/IL-2 therapy is effective in the treatment of biliary cancer.瞄准：为了示威，骨髓基质房间(BMSC ) 能在恶性病的治疗为基因修正被用作一个吸引人的目标。方法：用胆汁的癌症的一个仓鼠模型，我们调查了 interleukin-2 (IL-2 ) 的治疗学的效果修改基因的 BMSC。叙利亚的金色的仓鼠与 5 x 10 经由大腿骨的静脉被注射(5 ) KIGB-5 胆汁的癌症房间的房间排队(n=20 ) 。一个星期以后，仓鼠与仅仅包含 Ad/hIL-2 和 Ad/ 三角洲 E1，未修改的 BMSC，或 RPMI 的 BMSC (控制) intraperitoneally 被注射并且为 12 观察了 wk (n=5 /each 组) 。结果：与包含 Ad/hIL-2 的 BMSC 对待的所有仓鼠在这经期期间没有疾病的证据幸存。相反，在另外的三个组的仓鼠显示出象 4 wk 一样早包含肺的传播转移。结论：Ad/IL-2 治疗在胆汁的癌症的治疗是有效的。

关 键 词：Bone marrow stromal cell Adenovirus/hIL-2 Biliary cancer 

分 类 号：R735.8[医药卫生—肿瘤]