中性粘多糖对不同次缺血再灌流脑皮层中白三烯C_4含量的影响  

Effect of neutral mucoplysaccharide on in vitro release of LTC_4 of single and repetitive reperfusion ischemic gerbic cortex.

作　　者：管阳太[1] 郑惠民[2] 

机构地区：[1]上海医科大学神经病学研究所,200040 [2]上海长海医院神经内科

出　　处：《临床神经病学杂志》1996年第2期67-69,共3页Journal of Clinical Neurology

基　　金：上海市科委自然科学基金

摘　　要：本实验用放射免疫测定法观察了中性粘多糖对蒙古种沙土鼠单次和多次缺血再灌流脑皮层中白三烯 C_4(LTC_4)含量的影响。结果显示:缺血10分钟再灌流10分钟,并重复两次,较缺血30分钟再灌流30分钟的脑皮层在体外培养液中释放的 LTC_4量显著增加。缺血前10天,每天从胃管给予40 mg/kg 中性粘多糖后,多次缺血再灌流组 LT C_4释放量明显减少,而单次缺血再灌流组的 LT C_4释放量无明显变化。The ain of our study was to investigated the effect of neutral mucoplysac- charide(NM)on in vitro release of LTC4 of single and repetitive reperfusion ischemic cor- tex.Groups of gerbils underwent bilateral carotid occlusion and reperfusion.The single reper- fusion isehemie group(SRIG)underwent a single 30-Min period of carotid occlusion and 30- Min periol of reperfusion.The repetitive reperfusion ischemie group(RRIG)underwent three 10-Min periods of occlusion at 10-Min intervals.Other two groups were similar to SRIG and RRIG,respectively,but received 40 mg/kg NM each day for ten days before carotid occlusion.The LTC_4 level were determined by radioimmunoassay after 1-hour incu- bation of the cortical slices.The LTC_4 level in incubation of RRIG was considerably higher than that of SRIG.The increasing of LYO_4 of RRIG was blocked after pretreatment with NM,The results suggested NM might have some advantages on protection of ischemic reper- fusion injury of brain.

关 键 词：中性粘多糖 白三烯C4 脑缺血 再灌流损伤 

分 类 号：R743.31[医药卫生—神经病学与精神病学]