重组人骨形态发生蛋白-2诱导脂肪成体干细胞成异位软骨的研究  

Heterotopic chondrogenesis of adipose-derived adult stem cells induced by recombinant human bone morphogenetic protein-2

作　　者：魏义勇 胡蕴玉[1] 郝伟[1] 张德志[1] 吕荣[1] 白建萍[1] 王军[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710033

出　　处：《中华实验外科杂志》2006年第5期625-626,F0003,共3页Chinese Journal of Experimental Surgery

基　　金：全军医药卫生科研基金资助项目(01Z079)

摘　　要：目的探索重组人骨形态发生蛋白-2(rhBMP-2)诱导脂肪成体干细胞异位软骨的生成能力。方法将获取的兔脂肪组织机械分割,通过Ⅰ型胶原酶消化后得到脂肪成体干细胞,经 rhBMP-2诱导培养14d的脂肪成体干细胞微球种植在BMLB／C裸鼠肌袋内,术后4、8周各处死2 只动物,取材固定、脱钙、包埋后切片染色,镜下观察。结果经过rhBMP-2连续诱导培养14 d的脂肪成体干细胞已具有软骨细胞的表型(细胞基质中富含蛋白多糖和Ⅱ型胶原),并不向成骨方向分化(Von kossa染色阴性)。术后4、8周有软骨细胞形成。结论 rhBMP-2具有诱导脂肪成体干细胞向软骨细胞分化的潜能。Objective To investigate the possibility of heterotopic chondrogensis of adpose-derived adult stem cells induced by recombinant human bone morphogenetic protein-2. Methods Rabbit adipose tissue was minced and digested by collagenase type Ⅰ. The obtained adpose-derived adult stem cells were cultured in 10% FBS medium. Micropellets from harvested cells of adipose-derived adult stem cells were cultured in the medium containing recombinant human bone morphogenetic protein-2 at 14th day. After in vitro assay of chondrogenic phenotypes, the micropellets were implanted into 4 BALB/C athymic mice. Two mice were sacrificed at 4th and 8th week postoperatively and all samples were retrieved, fixed, embedded in paraffin and cut into 5μm-thick sections. HE staining was employed to observe chondrogenesis histologically. Results Alcian blue and immunohistochemical staining revealed chondroitin sulfate and collagen Ⅱ induced by rhBMP-2, but extracellular matrix mineral was not evident at 14th day postoperation. At 4th and 8th week postoperation heterotropic chondrogenesis was found in mice. Conclusion It seems that adipose-derived adult stem cell presents a potential for chondrogenic differentiation.

关 键 词：脂肪 干细胞 RHBMP-2 软骨细胞 

分 类 号：R68[医药卫生—骨科学]