在蔗糖预培养诱导耐脱水性过程中黄皮胚轴的水分状态和可溶性蛋白含量的变化  

Changes in Water State and Soluble Protein Contents of Wampee Axes during Inducing Desiccation Tolerance by Sucrose Preculture

作　　者：黄雪梅[1] 宋松泉[2] 傅家瑞[3] 

机构地区：[1]华南农业大学园艺学院,广州510642 [2]中国科学院西双版纳热带植物园,勐腊666303 [3]中山大学生命科学学院,广州510275

出　　处：《植物生理与分子生物学学报》2006年第2期245-251,共7页Journal Of Plant Physiology and Molecular Biology

基　　金：广东省自然科学基金项目(No.980360);科技部国家科技基础条件平台项目(No.2004DKA30430)资助。~~

摘　　要：逐步增加培养基的蔗糖浓度可提高黄皮胚轴的耐脱水性。用差示扫描量热仪(DSC)和SDS-PAGE方法探讨了黄皮胚轴在耐脱水性获得过程中的水分状态和可溶性蛋白的变化。DSC图谱显示,蔗糖预培养胚轴的升温图谱与对照不同,有比较明显的台阶式变化,有玻璃化形成的倾向;应用线性回归方程法或冰熔化热法计算黄皮胚轴的不可冻结水,发现蔗糖预培养胚轴的不可冻结水含量与对照胚轴无显著差异。蛋白分析表明,蔗糖预培养诱导黄皮胚轴中可溶性蛋白增加68%,其中尤以20kD蛋白的增幅最大。Progressively increasing sucrose concentration of culture medium could increase desiccation tolerance of wampee [Clausena lansium （Lour.） Skeels] axes. The changes in water state and soluble proteins of axes during acquirement of desiccation tolerance were measured by differential scanning calorimeter （DSC） and SDS-PAGE. The results showed that the cooling and heating thermograms of sucrose-precultured axes were similar to those of the control （Fig. 1, Fig.2）; but there was a stepwise change in the heating thermograms of sucrose-precultured axes, implying that vitrification might occur in axes （Fig.2）. Unfreezable water amounts of wampee axes were measured, the results showed that amounts of unfreezable water of sucrose-precultured axes and control were 25.4% and 25.9% （Table 1, by the linear regression equation method）, or 24.3% and 23.7% （by the heat of ice fusion method） respectively, which were not significantly different. The soluble protein content of sucrose-precultured axes was 68% higher than the control, and SDS-PAGE showed that a 20-kD protein markedly increased in content （Fig.3）.

关 键 词：黄皮胚轴 耐脱水性 水分状态 可溶性蛋白 

分 类 号：S666.6[农业科学—果树学]