人类角质形成细胞中调节细胞内信号传导的Toll样受体(TLR2)对糠秕马拉色菌的反应  

Toll-like receptor 2 (TLR2) mediates intracellular signalling in human keratinocytes in response to Malassezia furfur

作　　者：Baroni A. Orlando M. Donnarumma G. M.A. Tufano 刘艳 

机构地区：[1]Department of Experimental Medicine,Microbiology and Clinical Microbiology Section, Second University of Naples, via Costantinopoli no 16, 80138 Naples, Italy

出　　处：《世界核心医学期刊文摘（皮肤病学分册）》2006年第3期32-33,共2页Digest of the World Core Medical JOurnals:Dermatology

摘　　要：Toll-like receptors (TLRs) are crucial players in the innate immune response to microbial invaders. The lipophilic yeast Malassezia furfur has been implicated in the triggering of scalp lesions in psoriasis. The aim of the present study was to assess the role of TLRs in the defence against M. furfur infection. The expression of the myeloid differentiation factor 88 (MyD88) gene, which is involved in the signalling pathway of many TLRs, was also analysed. In addition, a possible correlation of antimicrobial peptides of the β -defensin family to TLRs was tested. Human keratinocytes infected with M. furfur and a variety oiM. furfur-positive psoriatic skin biopsies were analysed by RT-PCR, for TLRs, MyD88, human β -defensin 2 (HBD- 2), HBD- 3 and interleukin- 8 (IL- 8) mRNA expression. When keratinocytes were infected with M. furfur, an up-regulation for TLR2, MyD88, HBD- 2, HBD- 3 and IL- 8 mRNA was demonstrated, compared to the untreated cells. The same results were obtained when psoriatic skin biopsies were analysed. The M. furfur-induced increase in HBD- 2 and IL- 8 gene expression is inhibited by anti-TLR2 neutralising antibodies, suggesting that TLR2 is involved in the M. furfur-induced expression of these molecules. These findings suggest the importance of TLRs in skin protection against fungi and the importance of keratinocytes as a component of innate immunity.Toll-like receptors （TLRs） are crucial players in the innate immune response to microbial invaders. The lipophilic yeast Malassezia fuffur has been implicated in the triggering of scalp lesions in psoriasis. The aim of the present study was to assess the role of TLRs in the defence against M. fuffur infection. The expression of the myeloid differentiation factor 88 （MyD88） gene, which is involved in the signalling pathway of many TLRs, was also analysed. In addition, a possible correlation of antimierobial peptides of the β-defensin family to TLRs was tested. Human keratinocytes infected with M. furfur and a variety oiM. fur-fur-positive psoriatic skin biopsies were analysed by RT-PCR, for TLRs, MyD88, human β-defensin 2 （HBD- 2）, HBD - 3 and interleukin - 8 （IL - 8） mRNA expression. When keratinocytes were infected with M. furfur, an up-regulation for TLR2, MyD88, HBD-2,

关 键 词：糠秕马拉色菌感染 人类角质形成细胞 先天性免疫反应 TOU样受体 细胞内信号传导 白细胞介素-8(IL-8) RT-PCR检测 抗微生物肽 TLRS 调节 

分 类 号：R697.21[医药卫生—泌尿科学] R751[医药卫生—外科学]