尼美舒利对人SGC-7901细胞系增殖及hTERT-mRNA表达的研究  

Study the Effect of Nimesulide on Cell Proliferation of SGC-7901 Cell Line and Expression of hTERT-mRNA

作　　者：刘立玺[1] 石巍[2] 廖爱军[2] 文剑波[1] 文平[1] 

机构地区：[1]南方医科大学附属萍乡医院,337000 [2]南华大学附属第一医院消化内科,421000

出　　处：《实用癌症杂志》2006年第1期7-10,共4页The Practical Journal of Cancer

基　　金：湖南省卫生厅科学基金资助项目(Y02-074)

摘　　要：目的探讨选择性环氧合酶-2抑制剂尼美舒利对人胃癌细胞系SGC-7901增殖和端粒酶hTERT-mRNA表达的作用。方法体外培养人胃癌细胞系SGC-7901后,加入不同浓度的尼美舒利(0、50、100、200、400μmol/L)进行共同培养。用相差显微镜动态地观察经尼美舒利处理后细胞的形态变化;MTT法检测人胃癌细胞系SGC-7901的增殖;RT-PCR法检测细胞端粒酶hTERT-mRNA表达。结果选择性环氧合酶-2抑制剂尼美舒利作用于人胃癌细胞系SGC-7901后,细胞增殖受到明显的抑制,呈时间和剂量依赖性,并且端粒酶hTERT-mRNA的表达降低呈剂量依赖关系,与对照组比较差异有显著性(P<0.05).结论尼美舒利能够抑制人胃癌细胞系的hTERT-mRNA的表达,从而抑制其生长,这表明尼美舒利可能是种新的端粒酶抑制剂。Objective To study the effects of the selective COX-2 inhibitor（Nimesulide）on cell proliferation and expression of human telomerase reverse transcriptase messenger ribonucleic acid in the human gastric cancer cell line SGC-7901 and to explore the molecular mechanism of the antitumor activity of the selective COX-2 inhibitor. Methods After SGC-7901 was treated with different concentrations of Nimesulide（0,50,100,200,400μmol/L,） for different cultural time in vitro,the cellurlar proliferation was evaluated by MTT assay and the hTERT-mRNA expression was detected by RT-PCR. The cell morphology and growth manner was observed under phase contrant microscope. Results Nimesulide could inhibit the growth of SGC-7901 gastric cancer cell line in the time and dose-dependent manner;and the expression of hTERT-mRNA were decreased in dose-dependent manner; Compared with control group,it decreased significantly （P 〈 0, 05）, Conclusion Nimesulide decreased the expression of hTERT-mRNA in the gastrie cancer SGC-7901 cell line,these indicated Nimesulide is a probably telomerase inhibitor.

关 键 词：尼美舒利 SGC-7901细胞系 端粒酶催化亚单位 增殖 

分 类 号：R735.2[医药卫生—肿瘤]